Prostate Specific Antigen, Free Percentage (Includes Free PSA and Total PSA)
Ordering Recommendation

Do not use for initial prostate cancer screening; preferred test is Prostate Specific Antigen, Total (0070121) in conjunction with digital rectal exam (DRE). May provide additional prostate cancer risk information for patients with mildly elevated total PSA and a negative DRE.

Quantitative Electrochemiluminescent Immunoassay
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube or plasma separator tube. Also acceptable: Plain red, green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA). 
Specimen Preparation
Allow specimen to clot completely at room temperature. Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Grossly hemolyzed specimens. Vaginal washings. 
After separation from cells: Ambient: 8 hours; Refrigerated: 72 hours; Frozen: 3 months 
Reference Interval
By report
Results include: PSA-Free, PSA, PSA-Percent Free
Interpretive Data
The Roche PSA electrochemiluminescent immunoassay is used. Results obtained with different assay methods or kits cannot be used interchangeably. The Roche PSA method is approved for use as an aid in the detection of prostate cancer when used in conjunction with a digital rectal exam in men age 50 and older. The Roche Modular E170 PSA method is also indicated for the serial measurement of PSA to aid in the prognosis and management of prostate cancer patients. Elevated PSA concentrations can only suggest the presence of prostate cancer until biopsy is performed. PSA concentrations can also be elevated in benign prostatic hyperplasia or inflammatory conditions of the prostate. PSA is generally not elevated in healthy men or men with non-prostatic carcinoma.

ARUP uses the Roche Free PSA electrochemiluminescent immunoassay method in conjunction with the Roche PSA electrochemiluminescent immunoassay method to determine the free PSA percentage. The free PSA percentage is an aid in distinguishing prostate cancer from benign prostatic conditions in men age 50 and older with a total PSA between 3 and 10 ng/mL and negative digital rectal examination findings. Prostatic biopsy is required for the diagnosis of cancer. (Refer to: JAMA 1998; 279: 1542-1547)

In patients with total PSA concentrations of 4-10 ng/mL, the probability of finding prostate cancer on needle biopsy by age in years is:
% Free PSA
50-59 years
60-69 years
70 years and older
> 25 %9%12%16%
Other factors may help determine the actual risk of prostate cancer in individual patients.

The free percentage is calculated using the total and free PSA results.
Hotline History
Component Test Code*Component Chart NameLOINC
0070121Prostate Specific Antigen, Total83112-3
0080207Prostate Specific Antigen, Free10886-0
0080209Prostate Specific Antigen, Percent Free12841-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Free Prostate Specific Antigen
  • Free PSA
  • PSA Free/Total Ratio
  • PSA ratio
  • PSA Total and Free
  • PSA, Free
  • Total PSA