F-Actin (Smooth Muscle) Antibody, IgG by ELISA with Reflex to Smooth Muscle Antibody, IgG Titer
Ordering Recommendation
May be helpful in evaluating for autoimmune liver disease. For a more comprehensive reflex panel, refer to 2007210.
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube.(Min: 0.25 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Plasma. Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year  
Reference Interval
Test Number
Reference Interval
0055248F-Actin (Smooth Muscle) Antibody, IgGEffective May 16, 2011
19 Units or lessNegative
20-30 UnitsWeak Positive - Suggest repeat testing in two to three weeks with fresh specimen.
31 Units or greaterPositive - Suggestive of autoimmune hepatitis type 1 or chronic active hepatitis.

0051244Smooth Muscle Antibody, IgG TiterLess than 1:20 = Negative - No Antibody detected.
1:20-1:80 = Weak Positive - Suggest repeat testing in two to three weeks with fresh specimen.
1:160 or greater = Positive - Suggestive of autoimmune hepatitis or chronic active hepatitis.

Interpretive Data
Refer to report.

In viral hepatitis the titers are generally less than 1:80 and are transient. The titers in primary biliary cirrhosis are also low, ranging from 1:20 to 1:40.

If F-Actin is 20 Units or greater, then Smooth Muscle Ab, IgG IFA titer will be added. Additional charges apply
CPT Code(s)
83516; if reflexed, add 86256
Component Test Code*Component Chart NameLOINC
0055248F-Actin (Smooth Muscle) Ab, IgG by ELISA44706-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Actin Antibody
  • Actin Antibody Reflexive Panel
  • Anti-Smooth Muscle Antibody
  • ASMA
  • ASMA Reflexive Panel
  • F-Actin Antibody
  • F-Actin Antibody Panel
  • SMA
  • SMA IgG
  • SMA IgG Ab
  • SMA Reflexive Panel
  • Smooth Muscle Antibodies
  • Soft-SMA