Histoplasma Antibodies by CF
Ordering Recommendation
Semi-Quantitative Complement Fixation
1-3 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.25 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as acute or convalescent.  
Storage/Transport Temperature
Unacceptable Conditions
Contaminated or severely lipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
 Histoplasma Mycelia Antibody by CF Less than 1:8
 Histoplasma Yeast Antibody by CF Less than 1:8
Interpretive Data
An antibody titer greater than or equal to 1:8 is generally considered presumptive evidence of histoplasmosis. Greater than 1:32 or rising titers indicate strong presumptive evidence of histoplasmosis.

Yeast phase is regarded as more sensitive. Approximately 90-95 percent of cases have positive titers to one or both antigens. Titers to mycelial antigen are higher in chronic infection. Cross-reactions, usually at lower titers, may occur with other fungal disease. Rising titers suggest progression of infection. Skin tests in individuals previously exposed may cause titer elevation in 17-20 percent of cases.  
Negative fungal serology does not rule out the possibility of current infection.
CPT Code(s)
86698 x2
Component Test Code*Component Chart NameLOINC
0050330Histoplasma Mycelia, CF20573-2
0050335Histoplasma Yeast, CF20574-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • H. Mycelia Ab
  • Histoplasma Yeast Ab
  • Histoplasmosis Antibody