Histoplasma Antibodies by CF
Ordering Recommendation
Submit With Order
Semi-Quantitative Complement Fixation
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.25 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as acute or convalescent.  
Storage/Transport Temperature
Unacceptable Conditions
Contaminated or severely lipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
Histoplasma Mycelia Antibody by CF Less than 1:8
Histoplasma Yeast Antibody by CF Less than 1:8
Interpretive Data
An antibody titer greater than or equal to 1:8 is generally considered presumptive evidence of histoplasmosis. Greater than 1:32 or rising titers indicate strong presumptive evidence of histoplasmosis.

Yeast phase is regarded as more sensitive. Approximately 90-95 percent of cases have positive titers to one or both antigens. Titers to mycelial antigen are higher in chronic infection. Cross-reactions, usually at lower titers, may occur with other fungal disease. Rising titers suggest progression of infection. Skin tests in individuals previously exposed may cause titer elevation in 17-20 percent of cases.
Negative fungal serology does not rule out the possibility of current infection.
CPT Code(s)
86698 x2
Component Test Code*Component Chart Name
0050330Histoplasma Mycelia, CF
0050335Histoplasma Yeast, CF
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • H. Mycelia Ab
  • Histoplasma Yeast Ab