Hepatitis B Virus Surface Antigen with Reflex to Confirmation
Ordering Recommendation
Can be ordered as part of the acute hepatitis panel which includes HAV IgM, HBV core antibody IgM, HBV surface antigen, and HCV antibody. Refer to Hepatitis Panel, Acute with Reflex to HBsAg Confirmation (0020457).
Qualitative Chemiluminescent Immunoassay 
Within 24 hours  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL) Also acceptable: K2EDTA plasma.  
Storage/Transport Temperature
Unacceptable Conditions
Heparinized plasma. Specimens containing particulate material. Heat-inactivated, severely hemolyzed or lipemic specimens.  
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
 Hepatitis B Virus Surface Antigen Negative
0020128Hepatitis B Virus Surface Antigen, Confirmation Non Confirmed
Interpretive Data
This panel of assays should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).  
Performed and Reported times indicated are for screening of the HBsAg. If results for HBsAg screen are repeatedly reactive with an index value between 1.00 and 50.00, then HBsAg Confirmation will be added. Additional charges apply.
CPT Code(s)
87340; if reflexed, add 87341
Component Test Code*Component Chart NameLOINC
0020089Hepatitis B Surface Antigen5196-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Chronic Hep Screen B
  • HBsAg
  • HBSAg confirmation
  • HBV Surface Antigen
  • Hep B Surface Antigen
  • Hepatitis Bs Ag
  • Hepatitis Bs Ag Prenatal