Pentobarbital, Serum or Plasma
Ordering Recommendation

Optimize drug therapy and monitor patient adherence.

Quantitative Gas Chromatography/Mass Spectrometry
Wed, Sat
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum Pre-dose (Trough) Draw - At a Steady State Concentration or Plasma Pre-dose (Trough) Draw - At a Steady State Concentration in Plain Red, Lavender (K2EDTA), Lavender (K3EDTA), or Pink (K2EDTA). 
Specimen Preparation
Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Whole blood. Gel Separator Tubes, Light Blue (Sodium Citrate), or Yellow (SPS or ACD Solution). 
Please indicate in the supplied fields:
1.  Dose - List drug amount and include the units of measure
2.  Route - List the route of administration (IV, oral, etc.)
3.  Dose Frequency - Indicate how often the dose is administered (per day, per week, as needed, etc.)
4.  Type of Draw - Indicate the type of blood draw (Peak, Trough, Random, etc.) 
Ambient: 3 months; Refrigerated: 3 months; Frozen: 1 year 
Reference Interval
Therapeutic RangeSedation: 1-5 µg/mL
Intracranial Pressure (ICP) Therapy: 25-35 µg/mL
Coma: 10-50 µg/mL
Toxic LevelGreater than 10 µg/mL

Interpretive Data
The therapeutic range is based on serum pre-dose (trough) draw at steady-state concentration.Toxic concentrations may cause respiratory depression, hypotension, coma and death.

CPT Code(s)
Component Test Code*Component Chart NameLOINC
2011550Pentobarbital Dose
2011551Pentobarbital Dose Frequency
2011552Pentobarbital Route45373-8
2011553Pentobarbital Type of Draw49049-0
2011554Pentobarbital, Ser/Pla3924-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
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