Barbiturates, Serum or Plasma, Quantitative
Ordering Recommendation

Optimize drug therapy and monitor patient adherence. Useful for ruling out barbiturate exposure. For follow-up testing of a presumptive result, Barbiturates, Urine Quantitative (2012213) is preferred.

Quantitative Gas Chromatography-Mass Spectrometry
Tue, Thu, Sat
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Gray (sodium fluoride/potassium oxalate). Also acceptable: Plain red, green (sodium heparin), lavender (EDTA), or pink (K2EDTA). 
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 3.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1.5 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Separator tubes. Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles. Hemolyzed specimens. 
After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years 
Reference Interval
Drugs Covered
Cutoff Concentrations
Butalbital50 ng/mL
Amobarbital50 ng/mL
Pentobarbital50 ng/mL
Secobarbital50 ng/mL
Phenobarbital50 ng/mL

Interpretive Data
Methodology: Quantitative Gas Chromatography-Mass Spectrometry
Positive cutoff:
50 ng/mL

For medical purposes only; not valid for forensic use.

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
2012202Phenobarbital, S/P, Quant3948-7
2012203Butalbital, S/P, Quant6895-7
2012204Pentobarbital, S/P, Quant3924-8
2012205Amobarbital, S/P, Quant3338-1
2012206Secobarbital, S/P, Quant4028-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Amobarbital
  • Amobarbitone
  • Amytal
  • Axocet
  • Butalbital
  • Fioricet
  • Fiorinal
  • Luminal
  • Nembutal
  • Pain Management
  • Pentobarbital
  • Phenobarbital
  • Phenobarbitone
  • Quinalbartbitone
  • Sandoptal
  • Secobarbital
  • Seconal
  • Solfoton
  • Tuinal