2004328
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Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot

BORD BLOTS
Example Reports
Negative
Positive

Ordering Recommendation

If serology is used to assess late-stage pertussis (>4 weeks), the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774). CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).

New York DOH Approval Status

Testing is not New York state approved. Specimens from New York clients will be sent out to a New York state-approved laboratory.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)
New York State Clients: 0.5 mL (Min: 0.25 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Heat-inactivated specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
New York State Clients: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 1 month

Methodology

Qualitative Immunoblot

Performed

Tue, Fri

Reported

1-5 days

Reference Interval

Effective February 19, 2013

Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot
Components
 Reference Interval
Bordetella pertussis Antibody, IgG by Immunoblot
Bordetella pertussis Ab, IgG by Immunoblot Interp Negative
B. pertussis, IgG Immunoblot PT100 Negative
B. pertussis, IgG Immunoblot PT Negative
B. pertussis, IgG Immunoblot FHA Negative
Bordetella pertussis Antibody, IgA by Immunoblot
Bordetella pertussis Ab, IgA by Immunoblot Interp Negative
B. pertussis, IgA Immunoblot PT Negative
B. pertussis, IgA Immunoblot FHA Negative
Bordetella pertussis Antibody, IgM by Immunoblot
Bordetella pertussis Ab, IgM by Immunoblot Interp Negative
B. pertussis, IgM Immunoblot PT Negative
B. pertussis, IgM Immunoblot FHA Negative

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

This assay tests for the presence of pertussis toxin (PT), pertussis toxin PT 100 (PT-100), and filamentous hemagglutinin antibody (FHA).

Hotline History

N/A

CPT Codes

86615 x3

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Components

Component Test Code* Component Chart Name LOINC
2004317 B. pertussis Ab, IgA Immunoblot Interp 93479-4
2004318 B. pertussis, IgA Immunoblot PT 74765-9
2004319 B. pertussis Ab, IgG Immunoblot Interp 93479-4
2004320 B. pertussis, IgG Immunoblot FHA 69754-0
2004321 B. pertussis, IgG Immunoblot PT 69755-7
2004322 B. pertussis, IgG Immunoblot PT100 74766-7
2004323 B. pertussis Ab, IgM Immunoblot Interp 93479-4
2004324 B. pertussis, IgM Immunoblot FHA 43882-0
2004325 B. pertussis, IgM Immunoblot PT 69368-9
2004397 B. pertussis, IgA Immunoblot FHA 69753-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • B. pertussis Abs immunoblot
  • Pertussis Antibody
  • Pertussis IgA,IgG, IgM Abs Immunoblot
Bordetella pertussis Antibodies, IgA, IgG, and IgM by Immunoblot