Coccidioides Antibodies Panel, Serum by CF, ID, ELISA
Ordering Recommendation

Preferred serology test for detecting coccidioidomycosis (Valley fever).

Semi-Quantitative Complement Fixation/Qualitative Immunodiffusion/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Refer to individual components
2-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer two 1 mL aliquots of serum to individual ARUP Standard Transport Tube. (Min: 0.3 mL per aliquot) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent." 
Storage/Transport Temperature
Unacceptable Conditions
Other body fluids. Contaminated, hemolyzed, or severely lipemic specimens. 
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Effective November 18, 2013
Test Number
Reference Interval
0050170Coccidioides Antibody by CFLess than 1:2
0050183Coccidioides immitis Antibodies by ImmunodiffusionNone detected.
0050179Coccidioides Antibody, IgG by ELISA0.9 IV or less: Negative- No significant level of Coccidioides IgG antibody detected.
1.0-1.4 IV: Equivocal - Questionable presence of Coccidioides IgG antibody detected.  Repeat testing in 10-14 days may be helpful.
1.5 IV or greater: Positive - Presence of IgG antibody to Coccidioides detected, suggestive of current or past infection.
0050178Coccidioides Antibody, IgM by ELISA0.9 IV or less: Negative - No significant level of Coccidioides IgM antibody detected.
1.0-1.4 IV: Equivocal - Questionable presence of Coccidioides IgM antibody detected.  Repeat testing in 10-14 days may be helpful.
1.5 IV or greater: Positive - Presence of IgM antibody to Coccidioides detected, suggestive of current or recent infection.

Interpretive Data
Refer to individual components.

For Coccidioides, immunodiffusion (ID) measures IgM antibodies, while complement fixation (CF) measures both IgG and IgM. ELISA tests can be used to detect both coccidioidal IgG and IgM antibodies. While elevated single antibody titers may be diagnostic, paired specimens are preferred.  Acute and convalescent specimens (drawn at least 21 days apart), showing a fourfold or greater rise in titer, are diagnostic.

Negative fungal serology does not rule out the possibility of current infection.
Component Test Code*Component Chart NameLOINC
0050170Coccidioides Antibody by CF33380-7
0050178Coccidioides Antibody, IgM by ELISA33510-9
0050179Coccidioides Antibody, IgG by ELISA31154-8
0050183Coccidioides immitis Abs, Precipitin31153-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Cocci Antibodies Panel
  • Coccidioides Antibodies Serum panel
  • San Joaquin Fever Antibodies
  • Valley Fever Antibodies