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Hemoglobin Evaluation by HPLC With Reflex to Electrophoresis and/or RBC Solubility
Ordering Recommendation
Effective test to screen for hemoglobinopathies. May be used for follow up in individuals with a known hemoglobinopathy.
For hemoglobinopathy screening with reflex to molecular confirmation, see Hemoglobin Evaluation Reflexive Cascade (2005792).
New York DOH Approval Status
Specimen Required
Lavender (EDTA) or pink (K2EDTA).
Transport 5 mL whole blood in original tube. (Min: 0.5 mL) Also acceptable: whole blood in an ARUP standard transport tube.
Refrigerated
Frozen or room temperature specimens.
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable
Methodology
High Performance Liquid Chromatography (HPLC) / Capillary Electrophoresis / RBC Solubility
Performed
Sun-Sat
Reported
1-5 days
Reference Interval
| Test Number |
Components |
Reference Interval |
|---|---|---|
| Hemoglobin A | Age Reference Intervals (%) 0-1 months 7.6-54.8 2 months 14.7-70.1 3 months 26.6-81.8 4 months 43.0-89.5 5 months 60.8-94.0 6-8 months 78.2-96.6 9-12 months 86.1-97.2 13-23 months 85.1-97.7 2 years and older 95.0-97.9 |
|
| Hemoglobin A2 | Age Reference Intervals (%) 0-1 months 0.0-1.4 2 months 0.0-2.0 3 months 0.1-2.6 4 months 0.8-3.0 5 months 1.5-3.3 6-8 months 1.8-3.5 9-23 months 1.9-3.5 2 years and older 2.0-3.5 |
|
| Hemoglobin F | Age Reference Intervals (%) 0-1 months 45.8-91.7 2 months 32.7-85.2 3 months 14.5-73.7 4 months 4.2-56.9 5 months 1.0-38.1 6-8 months 0.9-19.4 9-12 months 0.6-11.6 13-23 months 0.0-8.5 2 years and older 0.0-2.1 |
|
| Hemoglobin S | 0.0 | |
| Hemoglobin C | 0.0 | |
| Hemoglobin E | 0.0 | |
| Hemoglobin Other | 0.0 |
Interpretive Data
Hemoglobin Evaluation:
HPLC Report: Results from HPLC are reported.
See CE Report: Results from capillary electrophoresis are reported under Hemoglobin Evaluation by Capillary Electrophoresis (3017102). Results were confirmed by HPLC.
Sickle Cell Solubility Reflex:
Not Performed: Solubility testing for hemoglobin S not indicated.
Positive: Positive for hemoglobin S by HPLC and confirmed by solubility testing.
Conf Previous: Positive for hemoglobin S by HPLC. Solubility testing performed previously and not repeated with this submission.
Hgb Capillary Electrophoresis Reflex:
Not Performed: Confirmation by capillary electrophoresis not indicated.
Performed: Results confirmed by capillary electrophoresis.
Conf Previous: Capillary electrophoresis confirmation performed as part of a previous submission. Confirmation not repeated with this submission.
Modified FDA
Note
If abnormal peaks suggestive of a hemoglobin variant are detected, then RBC solubility and/or capillary electrophoresis will be performed to aid in confirmation and identification of the variant. Additional charges apply.
If a hemoglobin variant cannot be quantitated by HPLC, results from capillary electrophoresis will be reported.
Quantitation of hemoglobin is recommended for a definitive diagnosis in infants 1 year and older.
Hotline History
Hotline History
CPT Codes
83021; if reflexed, add 83020; 85660
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 3005529 | Hemoglobin A | 4547-6 |
| 3005665 | Hemoglobin A2 | 4551-8 |
| 3005666 | Hemoglobin F | 4576-5 |
| 3005667 | Hemoglobin S | 4625-0 |
| 3005668 | Hemoglobin C | 4563-3 |
| 3005669 | Hemoglobin E | 4575-7 |
| 3005670 | Hemoglobin Other | 48343-8 |
| 3005671 | Hemoglobin Evaluation | 21026-0 |
| 3005673 | Sickle Cell Solubility Reflex | 6864-3 |
| 3017105 | Hgb Capillary Electrophoresis Reflex | 13514-5 |
Aliases
- Evaluation, Hemoglobin
- Fetal Hemoglobin (Hemoglobin F)
- Hb
- Hb ELP
- Hb IEF
- Hemoglobin Evaluation
- Hemoglobin Fractionation
- Hemoglobinopathy Fractionation Profile
- Hgb
- Sickle Cell Anemia Screen
- Sickle Cell Disease
- Sickle Cell Screen
