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FISH, Interphase, CD138+ Cells
Ordering Recommendation
Use to order individual or multiple FISH probes for patients with multiple myeloma.
New York DOH Approval Status
Specimen Required
Nondiluted bone marrow aspirate collected in a heparinized syringe. Also acceptable: Whole blood collected in green (sodium heparin) tube.
Transfer 3 mL bone marrow to a green (sodium heparin) (Min: 1 mL) tube. OR transport 5 mL whole blood (Min: 2 mL)
Room temperature.
Paraffin-embedded specimens. Clotted specimens.
Desired FISH probe and pertinent clinical diagnosis required with test order. Testing will not be performed until probe and diagnosis are provided ; absence of this information will delay turnaround time.
Clinical indication or reason for testing and specimen type required with test order. Sample will still be processed if this information is not initially provided but appropriate culturing and reporting may be compromised or delayed.
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Methodology
Fluorescence in situ Hybridization (FISH)
Performed
Sun-Sat
Reported
3-9 days
Reference Interval
Refer to report
Interpretive Data
Analyte Specific Reagent (ASR)
Note
Fluorescence in situ hybridization (FISH) is performed on CD138+ sorted cells (assuming specimen is sufficient for sorting) for multiple myeloma prognosis-specific genomic abnormalities. The comprehensive multiple myeloma panel is available under mnemonic FISHMMP (3002063). The following probes may be ordered individually or in any combination: 1p (CDKN2C) loss/deletion/1q (CKS1B) gain/amplification, 1q (CKS1B) gain/amplification/17p (TP53) loss/deletion, t(4;14) (IGH/FGFR3 and MMSET fusion), 8q (MYC) break apart, t(8;14) (IGH/MYC fusion), +9/9p (JAK2) trisomy/gain, t(11;14) (IGH/CCND1 fusion and/or +11), 13q (D13S319) deletion, 14q (IGH) break apart, t(14;16) (IGH/MAF fusion), t(14;20) (IGH/MAFB fusion), +15/15q,+17/17q (PML/RARA gain).
When this test is ordered in conjunction with a chromosome analysis, specimen prioritization will be given to FISH for the sorting of CD138+ cells. This could impact the successful completion of the chromosome analysis.
If sorting fails to yield sufficient CD138+ cells, testing will be performed using unsorted cells, if available.
If more than one probe is ordered, additional charges will apply.
Contact ARUP Genetics Processing (extension 3301) to add a probe to a current specimen.
Other specimen types may be acceptable, contact Genetics Processing (extension 3301) for specific specimen collection and transportation instructions.
This test must be ordered using Oncology test request form #43099 or through your ARUP interface.
If chromosome analysis is not performed at ARUP on the same sample, Bone Marrow/PBL Culture Processing Fee (0093271) will be added to account for sample processing, and additional charge will apply. If multiple FISH tests are ordered, 0093271 will only be applied to one of the FISH tests.
Hotline History
CPT Codes
88271; 88275
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0092615 | Chromosome FISH, Interphase | 57802-1 |
| 3002848 | EER Chromosome FISH, Interphase | 11526-1 |
Aliases
- +15/15q
- +17/17q (PML/RARA gain)
- 1p/1q
- 8q (MYC)
- CCND1/IGH (IGH/CCND1 fusion or +11)
- CD138
- CDKN2C (1p loss/deletion)
- CDKN2C/CKS1B
- CKS1B (1q gain)
- 13q (D13S319) deletion
- FGFR3/IGH
- 14q (IGH) break apart
- JAK2 (+9)
- MAF/IGH
- MAFB/IGH
- MGUS
- MM
- Multiple myeloma
- PCN
- Plasma cell neoplasms
- t(8;14) (IGH/MYC fusion)
- TP53 (17p deletion)
