PZA testing is discontinued by the manufacturer. ARUP will continue to test the other first-line drugs: rifampin, isoniazid, and ethambutol. If PZA susceptibility testing is requested, contact your local public health laboratory or the CDC for pncA gene testing for PZA resistance mutations.

0060999
print

Acid-Fast Bacillus (AFB) Identification

MC AFBID
Example Reports
Standard

Ordering Recommendation

Identify mycobacterial isolates, including requests for speciation within the M. tuberculosis complex.

If PZA susceptibility testing is requested for M. tuberculosis complex isolates, contact your local public health laboratory or the CDC for pncA gene testing for PZA resistance mutations. ARUP performs testing for other first-line drugs: rifampin, isoniazid, and ethambutol.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Actively growing organism, in pure culture.

Specimen Preparation

Place each specimen in an individually sealed bag. Transport sealed container with pure culture on solid or in liquid media.

Storage/Transport Temperature

Room temperature. If culture is suspected of being a microorganism identified on the IATA list as an infectious substance affecting humans, submit specimen according to Infectious Substance, Category A, shipping guidelines.

Unacceptable Conditions

Mixed cultures or non-viable organisms. Organisms submitted on an agar plate.

Remarks

Indicate source of organism and any other pertinent information, such as the growth rate of the organism.

Stability

Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks

Methodology

Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry/Polymerase Chain Reaction/Sequencing

Performed

Sun-Sat

Reported

Varies
Depends on organism growth.

Reference Interval

Complete identification of clinically significant isolates.

Interpretive Data

For identification by DNA sequencing and MALDI-TOF:

Compliance Category

Laboratory Developed Test (LDT)

Note

If the isolate M. tuberculosis complex is to be speciated, enter this information in the comment field and ship Category A.
A processing fee will be billed for all organisms submitted that are not in pure culture, as indicated in specimen requirements.
Identification of AFB isolates is billed by individual tests performed.

Hotline History

N/A

CPT Codes

CPT codes for identification vary based on method.

Are you an ARUP Client? Click here for your pricing.

Components

Component Test Code* Component Chart Name LOINC
0060999 AFB Identification 543-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • MTBGP
  • Mycobacterium tuberculosis Probe
  • AFB identification by sequencing, 16S rRNA
  • AFBSEQ
  • M. tuberculosis identification
Acid-Fast Bacillus (AFB) Identification