3021015
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Vitamin B3 (Niacin and Metabolites), Serum/Plasma

VIT B3
Example Reports
Abnormal
Normal

Ordering Recommendation

Use for nutritional assessment of vitamin B3 or to monitor therapeutic use of niacin.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Collect specimen after a 4-hour fast.

Collect

Plain Red, Serum Separator Tube (SST), Green (sodium or lithium heparin) or Plasma separator tube (PST)

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL of serum or plasma to an ARUP standard transport tube. (Min: 0.3 mL)

Separate specimens must be submitted when multiple tests are ordered.

Storage/Transport Temperature

Frozen

Unacceptable Conditions

Hemolyzed samples. Samples collected in EDTA tubes.

Remarks

Light protection is preferred during storage and shipment.

Stability

After separation from cells: Ambient: 6 hours; Refrigerated: 3 days; Frozen: 1 month

Methodology

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun, Tue, Wed, Fri, Sat

Reported

1-5 days

Reference Interval

Test Number
 Components
 Reference Interval
  Nicotinuric Acid Less than or equal to 55 nmol/L
  Nicotinamide 88-400 nmol/L
  Nicotinic Acid Less than or equal to 80 nmol/L

Interpretive Data

  Niacin (nicotinic acid, NIC) and its metabolites, nicotinamide (NAM) and nicotinuric acid (NUA) are measured in this assay. Measurable concentrations of NIC and NUA typically are not found in fasting specimens and may indicate therapeutic use of niacin.
Vitamin B3 concentrations are reported in nmol/L. To convert to nanograms per milliliter (ng/mL), multiply the reported concentration for nicotinamide by 0.122, nicotinic acid by 0.123, and nicotinuric acid by 0.180.

Compliance Category

Laboratory Developed Test (LDT)

Note

  For determination of vitamin B3 sufficiency, avoid use of vitamin supplements for 24 hours prior to collection.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
07/20/2026
X
N/A

CPT Codes

84591

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Components

Component Test Code* Component Chart Name LOINC
3021016 Nicotinic Acid 34342-6
3021017 Nicotinamide 25488-8
3021018 Nicotinuric Acid 72306-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Niacinamide
  • Niacor
  • Niaspan
  • Nicotinic Acid
  • Nicotinuric Acid
  • Slo-Niacin
Vitamin B3 (Niacin and Metabolites), Serum/Plasma