3020911
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  Hemoglobin Evaluation by HPLC and Electrophoresis With Reflex to RBC Solubility

HGBEL PLUS

Ordering Recommendation

Preferred test to screen for hemoglobinopathies. May be used for follow up in individuals with a known hemoglobinopathy.

For hemoglobinopathy screening with reflex to molecular confirmation, see Hemoglobin Evaluation Reflexive Cascade (2005792).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

  Lavender (EDTA) or pink (K2EDTA).

Specimen Preparation

  Transport 5 mL whole blood in original tube (Min: 0.5 mL). Also acceptable: whole blood in an ARUP standard transport tube.

Storage/Transport Temperature

  Refrigerated

Unacceptable Conditions

  Frozen or room temperature specimens.

Remarks
Stability

  Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable

Methodology

High Performance Liquid Chromatography (HPLC) / Capillary Electrophoresis / RBC Solubility

Performed

Sun-Sat

Reported

1-5 days

Reference Interval

Test Number
 Components
 Reference Interval
  Hemoglobin Other 0.0
  Hemoglobin E 0.0
  Hemoglobin C 0.0
  Hemoglobin S 0.0
  Hemoglobin F
Age Reference Intervals (%)
0-1 months 45.8-91.7
2 months 32.7-85.2
3 months 14.5-73.7
4 months 4.2-56.9
5 months 1.0-38.1
6-8 months 0.9-19.4
9-12 months 0.6-11.6
13-23 months 0.0-8.5
2 years and older 0.0-2.1

  Hemoglobin A
Age Reference Intervals (%)
0-1 months 7.6-54.8
2 months 14.7-70.1
3 months 26.6-81.8
4 months 43.0-89.5
5 months 60.8-94.0
6-8 months 78.2-96.6
9-12 months 86.1-97.2
13-23 months 85.1-97.7
2 years and older 95.0-97.9

  Hemoglobin A2
Age Reference Intervals (%)
0-1 months 0.0-1.4
2 months 0.0-2.0
3 months 0.1-2.6
4 months 0.8-3.0
5 months 1.5-3.3
6-8 months 1.8-3.5
9-23 months 1.9-3.5
2 years and older 2.0-3.5

Interpretive Data

  Hemoglobin Evaluation:
HPLC Report: Results from HPLC are reported. Results were confirmed by capillary electrophoresis.
See CE Report: Results from capillary electrophoresis are reported under Hemoglobin Evaluation by Capillary Electrophoresis (3017102). Results were confirmed by HPLC.
Sickle Cell Solubility Reflex:
Not Performed: Solubility testing for hemoglobin S not indicated.
Positive: Positive for hemoglobin S by HPLC and confirmed by solubility testing.
Conf Previous: Positive for hemoglobin S by HPLC. Solubility testing performed previously and not repeated with this submission.

Compliance Category

Modified FDA

Note

  HPLC and capillary electrophoresis are performed on all samples. If a hemoglobin variant cannot be quantitated by HPLC, results from capillary electrophoresis will be reported.
If abnormal peaks suggestive of a sickling hemoglobin variant are detected, then RBC solubility will be performed. Additional charges apply.

Hotline History

View Hotline History

 

Hotline History

Date of Change
Test Name Change
Methodology
Performance/Reported Schedule
Specimen Requirements
Reference Interval
Interpretive Data
Note
CPT Code
Component Change
Other Interface Change
New Test
Inactive
07/20/2026
X
N/A

CPT Codes

83021; 83020; if reflexed, add 85660

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Components

Component Test Code* Component Chart Name LOINC
3005529 Hemoglobin A 4547-6
3005665 Hemoglobin A2 4551-8
3005666 Hemoglobin F 4576-5
3005667 Hemoglobin S 4625-0
3005668 Hemoglobin C 4563-3
3005669 Hemoglobin E 4575-7
3005670 Hemoglobin Other 48343-8
3005673 Sickle Cell Solubility Reflex 6864-3
3020927 Hemoglobin Evaluation 21026-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Evaluation, Hemoglobin
  • Fetal Hemoglobin
  • Hemoglobin F
  • Hb
  • Hb ELP
  • Hb IEF
  • Hemoglobin Evaluation
  • Hemoglobin Fractionation
  • Hemoglobinopathy Fractionation Profile
  • Hgb
  • Sickle Cell Anemia Screen
  • Sickle Cell Disease
  • Sickle Cell Screen
  • Hemoglobinopathy
  • Hemoglobinopathies
Hemoglobin Evaluation by HPLC and Electrophoresis With Reflex to RBC Solubility