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Cell Culture for Genetic Testing
Ordering Recommendation
Processing test for culturing cells for genetic testing to be performed at ARUP, maintaining a frozen backup culture, and/or forwarding to an outside laboratory for additional genetic testing after initial ARUP testing is complete.
New York DOH Approval Status
Specimen Required
Amniotic fluid OR chorionic villi in cytogenetic tissue media (ARUP Supply #32788). If cytogenetic tissue media is not available, collect in plain RPMI, Hanks solution, sterile saline, or ringers.
New York State Clients: Discard first 2 mL of fluid aspirated to avoid maternal cell contamination.
Skin biopsy OR Product of Conception (POC): Place tissue in a sterile, screw-top container filled with thawed Cytogenetic Tissue Media (ARUP Supply #32788). Available online through eSupply using ARUP Connect. If Cytogenetic Tissue Media is not available, collect in plain RPMI, Hanks (HBSS), sterile saline, or ringers.
If autopsy is performed: Facia lata, diaphragm, tendon, skin, tissue from internal organs (if fresh), chest wall cartilage (particularly if macerated) or placenta from fetal side.
If no autopsy is performed: Placenta from fetal side is preferred (e.g., villi).
Also acceptable: Umbilical cord or achilles tendon.
Do not freeze any specimen submitted or expose to extreme temperatures. Do not place in formalin.
Amniotic fluid: Transport 30 mL amniotic fluid (min: 15 ml) in a sterile tube.
Chorionic Villi: 20 mg (min: 5mg) in a sterile, screw-top container filled with Cytogenetic Tissue Media.
New York State Clients: Specimen is collected in a 20 mL sterile syringe and transferred aseptically to sterile tubes. Specimen must be received at performing laboratory within 48 hours of collection.
Skin biopsy: Transport a 4 mm skin biopsy in a sterile, screw-top container filled with tissue transport media.
Product of Conception(POC): Transport POC, skin (POC), cord tissue, or decidua (min: 5mg) in sterile, screw-top container filled with tissue transport medium. If specimen size is too large for a normal collection tube, a larger sterile container can be used such as a sterile urine cup and can be flooded with several tubes of cytogenetic tissue media.
Cultured skin fibroblasts: 2 T-25 flasks at 80 percent confluency. Fill flasks with culture media. Backup cultures must be maintained at the client's institution until testing is complete.
Amniotic fluid, chorionic villi, skin OR POC sample: Room temperature.
Cultured skin fibroblasts: Critical room temperature.
Frozen specimens. Specimens preserved in formalin.
Amniotic fluid, chorionic villi, skin OR POC sample: Room Temperature: 2 days; Refrigerated: 2 days; Frozen: Unacceptable
Cultured skin fibroblasts: Room Temperature: 2 days; Refrigerated: Unacceptable; Frozen: Unacceptable
Methodology
Cell Culture
Performed
Sun-Sat
Reported
Not Applicable
Reference Interval
Interpretive Data
Not Applicable
Note
Desired tests must be indicated on the request form that accompanies the specimen. Cultured samples requested for frozen storage will be held for 6 months then discarded. Please note that contamination by maternal cells may interfere with attempts to culture cells and may cause interpretive problems.
Cell culturing should only be ordered in conjunction with testing at ARUP Laboratories OR for clients who utilize ARUP Laboratories as their primary cytogenetics laboratory. To confirm if it is appropriate to order cell culturing, please contact an ARUP genetic counselor at 800-242-2787 ext. 2141.
Cytogenetics Cell Culture for Genetic Testing includes a base cost for case management and review. Additional charges will apply if the following processing is required:
1. Culture Processing
3020744 AF/CVS
3020743 Skin/POC
2. Subculturing for total required flasks
3020735 AF/CVS 2 flasks
3020736 AF/CVS 3 flasks
3020944 AF/CVS 4 flasks
3020737 AF/CVS 5 flasks
3020947 AF/CVS 6 flasks
3020738 AF/CVS 7 flasks
3020949 AF/CVS 8 flasks
3020631 Tissue 2 flasks
3020721 Tissue 3 flasks
3020937 Tissue 4 flasks
3020723 Tissue 5 flasks
3020939 Tissue 6 flasks
3020728 Tissue 7 flasks
3020942 Tissue 8 flasks
3020729 Tissue 1 T25, 1 T75 flasks
3020730 Tissue 2 T25, 1 T75 flasks
3. 3020739 Cryopreservation
Hotline History
Hotline History
CPT Codes
88233, 88235, 88240
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 3020720 | Cell Culture for Genetic Testing |
Aliases
- Grow and Send
