Ordering Recommendation

Use to detect and quantitate RUNX1::RUNX1T1 fusions arising from t(8;21). Use to monitor for minimal residual disease (MRD) in patients with a known history of RUNX1::RUNX1T1 fusions arising from t(8;21) and assess the risk of disease relapse.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Whole blood or bone marrow in lavender (EDTA), pink (K2EDTA).

Specimen Preparation

Whole Blood: Transport 5 mL whole blood. (Min: 5 mL). Separate whole blood specimens must be submitted when multiple tests are ordered.
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Refrigerate immediately. Specimens must be received within 48 hours of collection due to lability of RNA.

Storage/Transport Temperature

Whole Blood or Bone Marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Serum, plasma, extracted DNA, CSF, FFPE tissue, ambient whole blood, or frozen whole blood or bone marrow.
Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.
Ambient bone marrow specimens past 7 days will be canceled.
Refrigerated whole blood or bone marrow specimens past 7 days will be canceled.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable

Methodology

Reverse Transcription Polymerase Chain Reaction

Performed

Varies

Reported

5-9 days

Reference Interval

Interpretive Data

Refer to report.

Compliance Category

Note

Hotline History

N/A

CPT Codes

81401

Components

Component Test Code* Component Chart Name LOINC
3020275 RUNX1::RUNX1T1, Source 66746-9
3020276 RUNX1::RUNX1T1, Result 21819-8
3020277 RUNX1::RUNX1T1, Ratio 72207-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • RUNX1::RUNX1T1 (AML1::ETO)
  • t(8;21) RUNX1::RUNX1T1 (AML1-ETO) t(8;21) Detection, Quantitative
  • AML1-ETO Q
RUNX1::RUNX1T1 ( AML1::ETO ) t(8;21) Detection, Quantitative