Ordering Recommendation

Use to process and hold DNA for future pretransplant recipient chimerism studies if there will be an extended delay between pretransplant sample collection and donor/posttransplant sample collection.

New York DOH Approval Status

This test is not New York state approved.

Specimen Required

Patient Preparation
Collect

Whole blood or bone marrow in lavender (EDTA), pink (K2EDTA), or yellow (ACD solution A or B). OR buccal brushes from recipient.

Specimen Preparation

Transport 2 mL whole blood (Min: 1 mL), OR 1 mL bone marrow (Min: 1 mL) refrigerated, OR 2 buccal brushes (cytology brushes) in a sterile, dry tube ambient. (Min: 2 brushes)

Storage/Transport Temperature

Whole blood: Refrigerated.
Buccal brush: Ambient.

Unacceptable Conditions

Whole blood, bone marrow, buccal swab, or saliva post transplant.
Plasma, serum

Remarks

Posttransplant results will be compared to pretransplant recipient and donor genotypes, therefore, donor and recipient specimens must be obtained and genotyped before the transplant even occurs. If transplant event occurred prior to specimen collection, dry buccal brushes (not bloody) are acceptable.

Stability

Whole Blood: Room temperature: 1 week; Refrigerated: 1 month; Frozen: Unacceptable
Buccal Brush: Room temperature: 1 week

Methodology

Quantitative Polymerase Chain Reaction (PCR)

Performed

Sun-Sat

Reported

Varies

Reference Interval

Interpretive Data



Compliance Category

Laboratory Developed Test (LDT)

Note

Extract and hold.

Hotline History

N/A

CPT Codes

N/A

Components

Component Test Code* Component Chart Name LOINC
3005450 Chimerism Recipient Pre, Specimen
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

Chimerism, Recipient, Pretransplant Process and Hold