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Somatic TP53 Mutations in Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue
Ordering Recommendation
Use to identify TP53 mutations in formalin-fixed, paraffin-embedded (FFPE) tissue from various tumors. TP53 mutation status is a prognostic biomarker in many tumor types, including lymphoid malignancies.
New York DOH Approval Status
Specimen Required
Tumor tissue.
New York State Clients: Paraffin-embedded tissue.
Formalin-fixed (10 percent neutral buffered formalin) and paraffin-embedded tissue.
Diff-Quik- and Papanicolaou-stained cytology smears are also acceptable. Number of slides needed for testing is dependent on the tumor cellularity of the smear. Slide(s) will be destroyed during testing process and will not be returned to client. Protect from excessive heat. Transport block and/or slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at 800-522-2787.
Resections: Transport 8 unstained 5-micron slides. (Min: 5 slides)
Small Biopsies: Transport 15 unstained 5-micron slides. (Min: 10 slides)
New York State Clients: Submit 7-10 unstained slides cut at 10 microns thick.
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
New York State Clients: Refrigerated. Also acceptable: Room temperature.
Less than 10 percent tumor. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens processed in a non-EDTA decalcifier. FNA smears with less than 50 tumor cells.
Include surgical pathology report.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block") or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Methodology
Qualitative Massively Parallel Sequencing
Performed
Varies
Reported
12-14 days
Reference Interval
Interpretive Data
Refer to report.
Note
Gene Tested: TP53 (NM_000546.5) exons 2-11
Hotline History
Hotline History
CPT Codes
81351, 81352
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 2002148 | Block ID | 57723-9 |
| 3017689 | TP53 FFPE Interp | 103680-5 |
