Ordering Recommendation

Aids in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is decreased in low-risk populations. May be used in persons who have received the bacille Calmette-Guérin (BCG) vaccine. 

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

QuantiFERON-TB Gold Plus (Standard) 4-Tube Collection Kit (ARUP Supply #54012) or QuantiFERON-TB Gold Plus (HIGH ALTITUDE) 4-Tube Collection Kit (ARUP Supply #54010) available online through eSupply using ARUP Connect(TM) or contact ARUP Client Services at 800-522-2787. Specimens may remain ambient for up to 16 hours after collection before being placed in an incubator. For collection and transport instructions refer to QuantiFERON under Special Handling at https://www.aruplab.com/testing/quantiferon#collection.

Specimen Preparation

Transport plasma in the original containers. (Min: 0.8 mL per container)

Storage/Transport Temperature


Unacceptable Conditions

Whole blood


Ambient: 2 hours; Refrigerated: 1 month; Frozen: Unacceptable


Semi-Quantitative Chemiluminescent Immunoassay (CLIA)/Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)




1-4 days

Reference Interval

Test Number
Reference Interval
  QuantiFERON Mitogen minus NIL
No Reference Interval

No Reference Interval

  Quantiferon Plus TB1 minus NIL
0.34 IU/mL or less

  Quantiferon Plus TB2 minus NIL
0.34 IU/mL or less

Interpretive Data

Interferon gamma release is measured for specimens from each of the four collection tubes. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values: NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). The NIL value represents nonspecific reactivity produced by the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB1-NIL tube specifically detects CD4+ lymphocyte reactivity, specifically stimulated by the TB1 antigens. The TB2-NIL tube detects both CD4+ and CD8+ lymphocyte reactivity, stimulated by TB2 antigens. An overall Negative result does not completely rule out TB infection.

A false-positive result in the absence of other clinical evidence of TB infection is not uncommon. Refer to: Updated Guidelines for Using Interferon Gamma Release Assays to Detect Mycobacterium tuberculosis Infection -- United States, 2010 (https://www.cdc.gov/mmwr/preview/mmwrhtml/rr5905a1.htm), for more information concerning test performance in low-prevalence populations and use in occupational screening.

Compliance Category



Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
3017623 QuantiFERON Mitogen minus NIL 71774-4
3017624 QuantiFERON NIL 71776-9
3017625 Quantiferon Plus TB1 minus NIL 64084-7
3017626 Quantiferon Plus TB2 minus NIL 88517-8
3017627 Quantiferon TB Gold Plus 71773-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


QuantiFERON TB-Gold Plus, 4-Tube