Ordering Recommendation

Use to determine the presence of hemoglobin S. Recommended test to detect sickle cell trait in athletes.

Methodology

High Performance Liquid Chromatography (HPLC)/RBC Solubility

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Lavender (EDTA) or pink (K2EDTA).

Specimen Preparation

Transport 5 mL whole blood. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Frozen or room temperature specimens.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable

Reference Interval

Negative

Interpretive Data

Negative: Negative for Hemoglobin S by HPLC. Solubility testing not performed.
Positive: Positive for Hemoglobin S by HPLC and confirmed by solubility testing. Additional charges apply.
Conf Previous: Positive for Hemoglobin S by HPLC. Solubility testing performed previously and not repeated with this submission.

This test does not differentiate hemoglobin S trait from homozygous sickle cell disease or other possible combinations such as: S/C, S/D, S/G, S/E, S/thalassemia, S/O-Arab, S/New York and C-Georgetown trait (Hb C-Harlem). For further clarification, Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility (ARUP test code 0050610) is recommended.

Compliance Category

FDA

Note

If HPLC detects a peak which suggests Hgb S, then RBC Solubility will be added for confirmation. Additional charges apply.

Hotline History

N/A

CPT Codes

83021; if reflexed, add 85660

Components

Component Test Code* Component Chart Name LOINC
3016617 Hemoglobin S Evaluation 4621-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Hb S
  • Hgb S
  • Sickle Cell Anemia Screen
  • Sickle Cell Disease
Hemoglobin S Evaluation with Reflex to RBC Solubility