Ordering Recommendation

Use to determine the presence of hemoglobin S. Recommended test to detect sickle cell trait in athletes.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Lavender (EDTA) or pink (K2EDTA).

Specimen Preparation

Transport 5 mL whole blood. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Frozen or room temperature specimens.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable

Methodology

High Performance Liquid Chromatography (HPLC)/RBC Solubility

Performed

Sun-Sat

Reported

1-4 days

Reference Interval

Negative

Interpretive Data

Negative: Negative for Hemoglobin S by HPLC. Solubility testing not performed.
Positive: Positive for Hemoglobin S by HPLC and confirmed by solubility testing. Additional charges apply.
Conf Previous: Positive for Hemoglobin S by HPLC. Solubility testing performed previously and not repeated with this submission.

This test does not differentiate hemoglobin S trait from homozygous sickle cell disease or other possible combinations such as: S/C, S/D, S/G, S/E, S/thalassemia, S/O-Arab, S/New York and C-Georgetown trait (Hb C-Harlem). For further clarification, Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility (ARUP test code 0050610) is recommended.

Compliance Category

FDA

Note

If HPLC detects a peak which suggests Hgb S, then RBC Solubility will be added for confirmation. Additional charges apply.

Hotline History

N/A

CPT Codes

83021; if reflexed, add 85660

Components

Component Test Code* Component Chart Name LOINC
3016617 Hemoglobin S Evaluation 4621-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Hb S
  • Hgb S
  • Sickle Cell Anemia Screen
  • Sickle Cell Disease
Hemoglobin S Evaluation with Reflex to RBC Solubility