Hemoglobin S Evaluation with Reflex to RBC Solubility
Ordering Recommendation
Use to determine the presence of hemoglobin S. Recommended test to detect sickle cell trait in athletes.
Methodology
High Performance Liquid Chromatography (HPLC)/RBC Solubility
Performed
Sun-Sat
Reported
1-4 days
New York DOH Approval Status
Specimen Required
Lavender (EDTA) or pink (K2EDTA).
Transport 5 mL whole blood. (Min: 0.2 mL)
Refrigerated.
Frozen or room temperature specimens.
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval
Negative
Interpretive Data
Negative: Negative for Hemoglobin S by HPLC. Solubility testing not performed.
Positive: Positive for Hemoglobin S by HPLC and confirmed by solubility testing. Additional charges apply.
Conf Previous: Positive for Hemoglobin S by HPLC. Solubility testing performed previously and not repeated with this submission.
This test does not differentiate hemoglobin S trait from homozygous sickle cell disease or other possible combinations such as: S/C, S/D, S/G, S/E, S/thalassemia, S/O-Arab, S/New York and C-Georgetown trait (Hb C-Harlem). For further clarification, Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility (ARUP test code 0050610) is recommended.
FDA
Note
If HPLC detects a peak which suggests Hgb S, then RBC Solubility will be added for confirmation. Additional charges apply.
Hotline History
Hotline History
CPT Codes
83021; if reflexed, add 85660
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
3016617 | Hemoglobin S Evaluation | 4621-9 |
Aliases
- Hb S
- Hgb S
- Sickle Cell Anemia Screen
- Sickle Cell Disease