Ordering Recommendation

Use to determine the cause of a prolonged prothrombin time (PT) or activated partial thromboplastin time (aPTT). A faculty pathologist personally directs each stage of testing to completion. A comprehensive interpretation is provided.

Condition-specific testing is preferred when the patient has a known coagulation disorder or a clear bleeding presentation.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation



At least five light blue (sodium citrate) tubes. Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.

Specimen Preparation

Transfer five 1 mL aliquots of platelet-poor plasma to five ARUP standard transport tubes and label as sodium citrate. (Min: 1 mL/aliquot and 5 mL total)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when additional tests codes are ordered.

Unacceptable Conditions

Anything other than sodium citrated plasma. Specimens containing anticoagulant medications. Clotted or hemolyzed specimens.


Submit the Patient History form for the Prolonged Clot Time Reflexive Profile.


Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen at -20C: 2 weeks


Electromagnetic Mechanical Clot Detection/Immunoturbidimetry/Microlatex Particle-Mediated Immunoassay/Platelet Agglutination/Chromogenic Assay




2-10 days

Reference Interval

Refer to individual components.

Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)


Submission of a completed Patient History form with test order will allow for optimal panel interpretation. The Patient History form for the Prolonged Clot Time Reflexive Profile is available on the ARUP web site or by contacting ARUP Client Services at 800-522-2787.

Initial testing will include D-Dimer (0030057), Fibrinogen (0030130), and Lupus Anticoagulant Reflex Panel (3017009). Depending on these initial findings, a pathologist will order one or more reflexive tests to provide a comprehensive interpretation. Additional testing may include Factor II, Activity (Prothrombin) (0030007); Factor V, Activity (0030075); Factor VII Activity (0030080), Factor VIII Activity (0030095), Chromogenic Factor VIII, Activity (3002343); Factor VIII Activity with Reflex to Bethesda Quantitative, Factor VIII (0030026); Factor IX, Activity (0030100); Factor IX Activity with Reflex to Bethesda Quantitative, Factor IX (0030032); Factor X, Activity (0030105); Factor XI, Activity (0030110); Factor XII, Activity (0030115); von Willebrand Factor Activity (Ristocetin Cofactor) (0030250); von Willebrand Factor Antigen (0030285); Fibrinogen Antigen (0030135); Inhibitor Assay, PT with Reflex to PT 1:1 Mix (2003260); and Inhibitor Assay, PTT with Reflex to PTT 1:1 Mix, with Reflex to 1-Hour Incubation (2003266). Additional charges apply.

Hotline History


CPT Codes

85390-26; additional CPT codes may apply: 85210; 85220; 85230; 85240; 85245; 85246; 85250; 85260; 85270; 85280; 85335; 85379; 85384; 85385; 85520; 85525; 85598; 85610; 85611; 85613; 85670; 85730; 85732.


Component Test Code* Component Chart Name LOINC
2014319 Prolonged Clot Time Reflex Panel Interp 48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Prolonged Clot Time Reflexive Profile