Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)
New York DOH Approval Status
Lavender ( K2EDTA plasma).
Conventional specimen preparation practices frequently do not fully remove platelets from plasma and contribute to elevated TGF-b measurements. Centrifuge anticoagulated whole blood within 2 hours of collection at 1500g for 10 minutes. Collect plasma and transfer to a fresh tube. Immediately centrifuge plasma at 3000g for 10 minutes. Collect plasma from upper 2/3 of tube without disturbing lower 1/3 of tube and transfer to fresh tube for storage or transport. Separate plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL plasma to an ARUP standard transport tube. (Min 0.3 mL)
CRITICAL FROZEN. Freeze <-20 C immediately. Separate specimens must be submitted when multiple tests are ordered.
Contaminated, severely hemolyzed, heat-inactivated or grossly lipemic specimens.
After separation from cells: Ambient: 30 minutes; Refrigerated: Unacceptable; Frozen: 60 days
Results are intended for research purposes or in attempts to understand the pathophysiology of unusual immune or inflammatory disorders.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|3005864||Transforming Growth Factor beta1, Plasma||49853-5|
- plasma TGFB1
- TGF-beta 1