Ordering Recommendation
Mnemonic
Methodology
Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)
Performed
Mon
Reported
1-8 days
New York DOH Approval Status
Specimen Required
Lavender ( K2EDTA plasma).
Conventional specimen preparation practices frequently do not fully remove platelets from plasma and contribute to elevated TGF-b measurements. Centrifuge anticoagulated whole blood within 2 hours of collection at 1500g for 10 minutes. Collect plasma and transfer to a fresh tube. Immediately centrifuge plasma at 3000g for 10 minutes. Collect plasma from upper 2/3 of tube without disturbing lower 1/3 of tube and transfer to fresh tube for storage or transport. Separate plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL plasma to an ARUP standard transport tube. (Min 0.3 mL)
CRITICAL FROZEN. Freeze <-20 C immediately. Separate specimens must be submitted when multiple tests are ordered.
Contaminated, severely hemolyzed, heat-inactivated or grossly lipemic specimens.
After separation from cells: Ambient: 30 minutes; Refrigerated: Unacceptable; Frozen: 60 days
Reference Interval
1654-19951 pg/mL
Interpretive Data
Results are intended for research purposes or in attempts to understand the pathophysiology of unusual immune or inflammatory disorders.
Laboratory Developed Test (LDT)
Note
Hotline History
Hotline History
CPT Codes
83520
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
3005864 | Transforming Growth Factor beta1, Plasma | 49853-5 |
Aliases
- Cytokine
- cytokines
- plasma TGFB1
- TGF-b
- TGF-beta 1
- TGFb
- TGFB-1
- TGFbeta1