Ordering Recommendation

Use as a sensitive test to detect members of the orthopoxviruses, including monkeypox virus and vaccinia virus.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Lesion swab. Swab the lesion vigorously to collect adequate DNA. It is not necessary to deroof the lesion before swabbing.

Specimen Preparation

Place swab in Viral Transport Media (ARUP supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787

Storage/Transport Temperature


Unacceptable Conditions

Calcium alginate swab, wooden swab. Specimens without swabs.


Specimen source required.


Ambient: 24 hours; Refrigerated: 7 days; Frozen: 1 month


Qualitative Polymerase Chain Reaction




1-3 days

Reference Interval

Interpretive Data

This assay does not differentiate members of the orthopoxviruses. In the United States, a detected result is most likely due to monkeypox virus or vaccinia virus. Other orthopoxviruses may be considered if appropriate. Refer to the US Centers for Disease Control and Prevention if additional confirmatory testing is needed.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


This test is intended for the detection of non-variola orthopoxviruses, however high viral titer variola virus (smallpox) infections could be detected by this assay. Smallpox was declared eradicated in 1980 by the World Health Organization and the last case in humans was described in 1977.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
3005717 Orthopoxvirus Source 31208-2
3005719 Orthopoxvirus by PCR 41853-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Monkeypox
  • non-variola orthopoxvirus
  • orthopoxvirus, non-variola
  • vaccinia
Orthopoxvirus (includes monkeypox virus) by PCR