Use as a sensitive test to detect members of the orthopoxviruses, including monkeypox virus and vaccinia virus.
Qualitative Polymerase Chain Reaction
New York DOH Approval Status
Lesion swab. Swab the lesion vigorously to collect adequate DNA. It is not necessary to deroof the lesion before swabbing.
Place swab in Viral Transport Media (ARUP supply #12884) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787
Calcium alginate swab, wooden swab. Specimens without swabs.
Specimen source required.
Ambient: 24 hours; Refrigerated: 7 days; Frozen: 1 month
This assay does not differentiate members of the orthopoxviruses. In the United States, a detected result is most likely due to monkeypox virus or vaccinia virus. Other orthopoxviruses may be considered if appropriate. Refer to the US Centers for Disease Control and Prevention if additional confirmatory testing is needed.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
This test is intended for the detection of non-variola orthopoxviruses, however high viral titer variola virus (smallpox) infections could be detected by this assay. Smallpox was declared eradicated in 1980 by the World Health Organization and the last case in humans was described in 1977.
|Component Test Code*||Component Chart Name||LOINC|
|3005719||Orthopoxvirus by PCR||41853-3|
- non-variola orthopoxvirus
- orthopoxvirus, non-variola