Ordering Recommendation

Use when a diagnosis of essential thrombocythemia (ET) or primary myelofibrosis (PMF) is suspected.

Mnemonic

ETPMF RFX

Methodology

Droplet Digital Polymerase Chain Reaction/Capillary Electrophoresis

Performed

DNA Isolation: Sun-Sat
Assay: Varies

Reported

3-15 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Whole blood or bone marrow: Lavender (EDTA), preferred. Also acceptable: Green (sodium heparin)

Specimen Preparation

Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow:
Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, serum, FFPE tissue blocks/slides, or fresh or frozen tissue, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.

Remarks
Stability

Refrigerated: 7 days; Frozen: Unacceptable

Reference Interval

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

If JAK2 QUAL is reported as "Not Detected" then CALR Exon 9 Mutation Analysis by PCR will be added. If CALR is reported as "Not Detected," then MPL Mutation Detection will be added. Additional charges apply.

Hotline History

N/A

CPT Codes

81270; if reflexed add 81219; if reflexed again add 81338

Components

Component Test Code* Component Chart Name LOINC
3004047 JAK2 QUAL, Source 31208-2
3004048 JAK2 QUAL Mutation by PCR 43399-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • CALR
  • MPL
JAK2 (V617F) Mutation by ddPCR, Qualitative with Reflex to CALR (Calreticulin) Exon 9 Mutation Analysis by PCR with Reflex to MPL Mutation Detection