Ordering Recommendation

Use when a diagnosis of essential thrombocythemia (ET) or primary myelofibrosis (PMF) is suspected.




Droplet Digital PCR (ddPCR)/Capillary Electrophoresis


DNA Isolation: Sun-Sat
Assay: Varies


3-15 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Whole blood or bone marrow: Lavender (EDTA).

Specimen Preparation

Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow:
Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)

Storage/Transport Temperature


Unacceptable Conditions

Plasma, serum, FFPE tissue blocks/slides, or fresh or frozen tissue. Specimens collected in anticoagulants other than EDTA. Clotted or grossly hemolyzed specimens.


Refrigerated: 7 days; Frozen: Unacceptable

Reference Interval

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


If JAK2 QUAL is reported as "Not Detected" then CALR Exon 9 Mutation Analysis by PCR and MPL Mutation Detection will be added. Additional charges apply.

Hotline History


CPT Codes

81270; if reflexed add 81219; if reflexed again add 81338


Component Test Code* Component Chart Name LOINC
3004047 JAK2 QUAL, Source 31208-2
3004048 JAK2 QUAL Mutation by PCR 43399-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • CALR
  • MPL
JAK2 (V617F) Mutation by ddPCR, Qualitative with Reflex to CALR (Calreticulin) Exon 9 Mutation Analysis by PCR with Reflex to MPL Mutation Detection