Not a recommended first-line screening test for ANCA-associated vasculitis. Detection of ANCA should be followed by testing for MPO-ANCA/PR3-ANCA to determine antibody specificity; ANCA may be positive in the absence of PR3-ANCA and MPO-ANCA in a number of other systemic or inflammatory diseases.
Semi-Quantitative Indirect Fluorescent Antibody
New York DOH Approval Status
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)
Plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|ANCA IFA Titer||Less than 1:20|
|ANCA IFA Pattern||None Detected|
Neutrophil Cytoplasmic Antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90 percent of patients with certain necrotizing systemic vasculitides, and usually in less than 5 percent of patients with collagen vascular disease or arthritis.
ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
|Component Test Code*||Component Chart Name||LOINC|
|3003764||ANCA IFA Pattern||21419-7|
|3003765||ANCA IFA Titer||29967-7|
- ANCA IgG
- ANCA IgG Ab