Ordering Recommendation

Not a recommended first-line screening test for ANCA-associated vasculitis. Detection of ANCA should be followed by testing for MPO-ANCA/PR3-ANCA to determine antibody specificity; ANCA may be positive in the absence of PR3-ANCA and MPO-ANCA in a number of other systemic or inflammatory diseases.

Mnemonic

ANCA-IFA

Methodology

Semi-Quantitative Indirect Fluorescent Antibody

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

ANCA IFA Titer Less than 1:20
ANCA IFA Pattern None Detected

Interpretive Data

Neutrophil Cytoplasmic Antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90 percent of patients with certain necrotizing systemic vasculitides, and usually in less than 5 percent of patients with collagen vascular disease or arthritis.

Compliance Category

FDA

Note

ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.

Hotline History

N/A

CPT Codes

86255

Components

Component Test Code* Component Chart Name LOINC
3003764 ANCA IFA Pattern 21419-7
3003765 ANCA IFA Titer 29967-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • ANCA
  • ANCA IgG
  • ANCA IgG Ab
Anti-Neutrophil Cytoplasmic Antibody, IgG by IFA