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Anti-Neutrophil Cytoplasmic Antibody, IgG by IFA
Ordering Recommendation
Not a recommended first-line screening test for ANCA-associated vasculitis. Detection of ANCA should be followed by testing for MPO-ANCA/PR3-ANCA to determine antibody specificity; ANCA may be positive in the absence of PR3-ANCA and MPO-ANCA in a number of other systemic or inflammatory diseases.
Methodology
Semi-Quantitative Indirect Fluorescent Antibody (IFA)
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
Specimen Required
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)
Refrigerated.
Plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval
| ANCA IFA Titer | Less than 1:20 |
| ANCA IFA Pattern | None Detected |
Interpretive Data
Neutrophil Cytoplasmic Antibodies (C-ANCA = granular cytoplasmic staining, P-ANCA = perinuclear staining) are found in the serum of over 90 percent of patients with certain necrotizing systemic vasculitides, and usually in less than 5 percent of patients with collagen vascular disease or arthritis.
FDA
Note
ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
Hotline History
CPT Codes
86036
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 3003764 | ANCA IFA Pattern | 21419-7 |
| 3003765 | ANCA IFA Titer | 29967-7 |
Aliases
- ANCA
- ANCA IgG
- ANCA IgG Ab
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