Ordering Recommendation

Use to optimize drug therapy and monitor patient adherence.


Quantitative Liquid Chromatography-Tandem Mass Spectrometry




1-8 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Timing of specimen collection: Predose (trough) draw - At steady state concentration.


Plain red. Also acceptable: Lavender (K2 or K3EDTA), pink (K2EDTA), green (Heparin) or gray (Potassium oxalate or Sodium Fluoride).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions

Whole blood. Gel separator tubes, light blue (citrate), or yellow (SPS or ACD solution).


After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 14 days

Reference Interval

Bupropion Hydroxybupropion
Therapeutic Range 10-100 ng/mL 850-1500 ng/mL
Toxic Level Greater than or equal to 400 ng/mL Greater than or equal to 2000 ng/mL

Interpretive Data

Bupropion is an antidepressant drug indicated for the treatment of major depressive disorder. The drug is also used as treatment for smoking cessation. The therapeutic range is based on serum predose (trough) draw at steady-state concentration. Bupropion is primarily metabolized to hydroxybupropion, which has about 50 percent of the activity of the parent drug. The pharmacokinetics of bupropion and metabolite are influenced by drug-drug interactions that affect CYP2B6 metabolism. Patients with renal or hepatic impairment may require a dose reduction. Adverse effects may include seizures, hypertension, nausea, vomiting, neuropsychiatric and cardiac abnormalities.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes

80338 (Alt code: G0480)


Component Test Code* Component Chart Name LOINC
0099184 Bupropion 6706-6
3003038 Hydroxybupropion 9418-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Wellbutrin (Bupropion)
  • Zyban (Bupropion)
Bupropion and Metabolite, Serum or Plasma