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Epstein-Barr Virus (EBV) by in situ Hybridization on Paraffin
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Ordering Recommendation
Use to detect Epstein-Barr virus in paraffin. Includes interpretation.
Mnemonic
Methodology
In situ hybridization (ISH)
Performed
Mon-Fri
Reported
2-5 days
New York DOH Approval Status
Specimen Required
Tissue.
Formalin fix (10% neutral buffered formalin) and paraffin-embed tissue. Transport tissue block or 5 unstained 5 micron slides. (Min: 4 slides) Protect paraffin block and/or slides from excessive heat.
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Specimens fixed or processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5).
Include surgical pathology report.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Reference Interval
Negative
Interpretive Data
Refer to report
Laboratory Developed Test (LDT)
Note
Hotline History
CPT Codes
88365
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 3003235 | H and E Slide Description | |
| 3003236 | Epstein-Barr Virus (EBV) ISH Result | |
| 3003237 | EBV ISH Comment | |
| 3003240 | ISH Reference Number | |
| 3003241 | ISH Tissue Source |
Aliases
- EBER ISH
- EBV by ISH
- EBV ISH
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