Ordering Recommendation

Aids in the diagnosis of mastocytosis. Provides prognostic and predictive information for tyrosine kinase inhibitor (TKI) therapy planning.

Mnemonic
KITD816V Q
Methodology

Droplet Digital Polymerase Chain Reaction

Performed

DNA isolation: Sun-Sat
Assay:
Varies

Reported

2-7 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Whole blood or bone marrow: Lavender (EDTA), preferred. Also acceptable: Green (sodium heparin)

Specimen Preparation

Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow:
Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, serum, FFPE tissue blocks/slides, or fresh or frozen tissue, DNA extracted by a non-CLIA certified lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.

Remarks
Stability

Refrigerated: 7 days; Frozen: Unacceptable

Reference Interval
Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

81273

Components
Component Test Code* Component Chart Name LOINC
3002961 KIT QNT, Source 31208-2
3002962 KIT D816V Variant Allele Frequency 81258-6
3002963 KIT D816V Mutation by PCR 88519-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Asp816Val
  • C-KIT
  • CKIT
  • D816V
  • KIT exon 17
KIT (D816V) Mutation by ddPCR, Quantitative