Ordering Recommendation
Preferred test for detecting antibodies during acute or convalescent phase. Convalescent sera may be required for diagnosis.
Mnemonic
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Mon, Wed, Fri
Reported
1-6 days
New York DOH Approval Status
Specimen Required
Serum separator tube (SST) or Plain Red/Red-Top
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)
Refrigerated.
Contaminated, heat-inactivated, or turbid specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month
Reference Interval
Test Number |
Components |
Reference Interval |
---|---|---|
Francisella tularensis Antibody, IgG | 9 U/mL or less | |
Francisella tularensis Antibody, IgM | 9 U/mL or less |
Interpretive Data
Cross- reactivity with Brucella and Yersinia antibodies may occur. Therefore, results should be interpreted with caution and correlated with clinical information. The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are performed in the same laboratory at the same time.
Component | Interpretation |
---|---|
Francisella tularensis Antibody, IgG | 9 U/mL or less Negative - No significant level of IgG antibody to Francisella tularensis detected. 10-15 U/mL Equivocal - Questionable presence of IgG antibody to Francisella tularensis. Repeat testing in 10-14 days may be helpful. 16 U/mL or greater Positive - Presence of IgG antibody to Francisella tularensis detected, suggestive of current or past exposure/immunization. |
Francisella tularensis Antibody, IgM | 9 U/mL or less Negative - No significant level of IgM antibody to Francisella tularensis detected. 10-15 U/mL Equivocal - Questionable presence of IgM antibody to Francisella tularensis. Repeat testing in 10-14 days may be helpful. 16 U/mL or greater Positive - Presence of IgM antibody to Francisella tularensis detected, suggestive of current or recent exposure/immunization |
Laboratory Developed Test (LDT)
Note
If the ELISA testing is equivocal or positive for IgG and/or IgM, then Francisella tularensis Antibodies by Agglutination will be added. Additional charges apply.
Hotline History
CPT Codes
86668 x2, if reflexed add 86000
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2005351 | Francisella tularensis Antibody, IgG | 70121-9 |
2005352 | Francisella tularensis Antibody, IgM | 16878-1 |
Aliases
- F. tularensis IgG, IgM
- Febrile Antigens
- Francisella Antibodies
- Francisella Tularensis Ab