Recommended autoantibody panel for the evaluation of individuals with suspected primary biliary cholangitis (PBC).
Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
Serum Separator Tube (SST).
Separate from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)
Non-serum, heat-inactivated, contaminated, grossly icteric, severely lipemic, grossly hemolyzed specimens or inclusion of fibrin clot.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month
|0050065||Mitochondrial M2 Antibody, IgG (ELISA)||
|3000082||Antinuclear Antibody (ANA) with HEp-2 Substrate, IgG by IFA||Less than 1:80|
|3002478||Anti-sp100 Antibody, IgG||
|3002477||Anti-gp210 Antibody, IgG||
Refer to report.
ANA identified by indirect fluorescence assay (IFA) using HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. Positive nuclear patterns reported include homogeneous, speckled, centromere, nucleolar, or nuclear dots. Positive cytoplasmic patterns reported include reticular/AMA, discrete/GW body-like, polar/golgi-like, rods and rings, or cytoplasmic speckled patterns. All positive results are reported with endpoint titers at no additional charge.
83516 x3, 86039
|Component Test Code*||Component Chart Name||LOINC|
|0050065||Mitochondrial (M2) Antibody, IgG||14251-3|
|3000090||Antinuclear Antibody (ANA), HEp-2, IgG||21424-7|
|3000096||ANA Interpretive Comment||49311-4|
|3002483||Anti-gp210 Antibody, IgG|
|3002484||Anti-sp100 Antibody, IgG|