Ordering Recommendation

First-line testing for connective tissue disease.

Mnemonic
CTD PAN
Methodology

Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody (IFA)/Semi-Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Specimen types other than those listed. Specimens containing fibrin clots. Contaminated, grossly hemolyzed, heat-inactivated, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
0050215 Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA Effective August 20, 2012
Test Number
Components
Reference Interval
Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA None Detected.
2002693 Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) Less than 1:10

2002693 Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) Less than 1:10
0050470 Smith/RNP (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

0050085 Smith (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

2012074 SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
Test Number
Components
Reference Interval
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

0050692 SSB (La) (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

0099592 Jo-1 Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

0050599 Scleroderma (Scl-70) (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

Interpretive Data

Refer to report.

Compliance Category

FDA

Note

If Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA is detected, then Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) will be added. Additional charges apply.

Hotline History
N/A
CPT Codes

86235 x7 and 86225; if reflexed, add 86256

Components
Component Test Code* Component Chart Name LOINC
0050085 Smith (ENA) Antibody, IgG 18323-6
0050215 Double-Stranded DNA (dsDNA) Ab IgG ELISA 58465-6
0050470 Smith/RNP (ENA) Ab, IgG 33983-8
0050599 Scleroderma (Scl-70) (ENA) Antibody, IgG 47322-3
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
0050692 SSB (La) (ENA) Antibody, IgG 29965-1
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • dsDNA
  • RNP
  • Smith
  • SSA
  • SSB IgG
Connective Tissue Disease First Line Panel with Reflex