1p19q Deletion by FISH and IDH1 R132H Point Mutation by Immunohistochemistry with Reflex to IDH1 and IDH2 Mutation Analysis, Exon 4
Preferred initial test for the diagnosis of oligodendrogliomas. Detect IDH1 or IDH2 mutation and 1p/19q co-deletion, which are both necessary for a firm diagnosis of oligodendrogliomas.
Fluorescence in situ Hybridization/Immunohistochemistry/Polymerase Chain Reaction/Sequencing
1-7 days, add 8-14 days if reflexed
New York DOH Approval Status
Formalin fix (10 percent neutral buffered formalin is preferred) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or 10 unstained (5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800)522-2787. (Min. 6 slides) If sending precut slides, do not oven bake.
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Paraffin block with less than 25 percent tumor tissue. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.
Include surgical pathology report.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Refer to report.
Laboratory Developed Test (LDT)
This test code includes pathologist interpretation.
Negative IDH1 IHC results will reflex to IDH1 and IDH2 Mutation Analysis, Exon 4, to assess for less common IDH mutations. Additional charges apply.
88342, 88377 x2, if reflexed add 88381; 81120; 81121
|Component Test Code*||Component Chart Name||LOINC|
|2007358||IDH1 R132H Mutation Reference Number|
|3002136||IDH1 R132H Point Mut by IHC with Reflex|
|3003120||IDH1 Tissue Source|
- 1p,1q codeletion FISH
- 1p/19q Deletion
- 1p/19q Deletion in Gliomas, FISH, Tissue
- 1p/19q deletion in Oligodendroglioma
- 1p/19q FISH
- 1p/19q in oligodendrogliomas
- 1q deletion FISH
- Astrocytoma and Oligodendroglioma Tumor Cell Marker
- Chromosome Analysis by FISH for 1p/19q Deletion in Gliomas
- FISH for 1p/19q Deletion in Gliomas
- IDH1 Point mutation IHC
- IDH1 R132H antibody staining
- IDH1 R132H IHC, Isocitrate dehydrogenase, 1p deletion FISH
- LOH (Loss of Heterozygosity) 1p/19q in Gliomas
- Loss of Heterozygosity (LOH) 1p/19q in Gliomas