Ordering Recommendation

Preferred initial test for the diagnosis of oligodendrogliomas. Detect IDH1 or IDH2 mutation and 1p/19q co-deletion, which are both necessary for a firm diagnosis of oligodendrogliomas. Negative IDH1 IHC results will reflect to IDH1 and IDH2 Mutation Detection (3017222) to assess for less common IDH mutations. 

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Tumor tissue.

Specimen Preparation

Formalin fix (10 percent neutral buffered formalin is preferred) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or 10 unstained (5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800)522-2787. (Min. 6 slides) If sending precut slides, do not oven bake.

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Paraffin block with less than 25 percent tumor tissue. Specimens fixed in any fixative other than 10 percent neutral buffered formalin. Decalcified specimens.


Include surgical pathology report.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.


Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable


Fluorescence in situ Hybridization (FISH)/Immunohistochemistry/Polymerase Chain Reaction/Sequencing




1-7 days
If reflexed, add 8-14 days.

Reference Interval

Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)


This test code includes pathologist interpretation.

Negative IDH1 IHC results will reflex to IDH1 and IDH2 Mutation Analysis, Exon 4, to assess for less common IDH mutations. Additional charges apply.

Hotline History


CPT Codes

88342; 88377 x2; if reflexed, add 81120; 81121


Component Test Code* Component Chart Name LOINC
2007358 IDH1 R132H Mutation Reference Number 94736-6
3002136 IDH1 R132H Point Mut by IHC with Reflex
3003120 IDH1 Tissue Source 31208-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • 1p,1q codeletion FISH
  • 1p/19q Deletion
  • 1p/19q Deletion in Gliomas, FISH, Tissue
  • 1p/19q deletion in Oligodendroglioma
  • 1p/19q FISH
  • 1p/19q in oligodendrogliomas
  • 1q deletion FISH
  • Astrocytoma and Oligodendroglioma Tumor Cell Marker
  • Chromosome Analysis by FISH for 1p/19q Deletion in Gliomas
  • FISH for 1p/19q Deletion in Gliomas
  • IDH1 Point mutation IHC
  • IDH1 R132H antibody staining
  • IDH1 R132H IHC, Isocitrate dehydrogenase, 1p deletion FISH
  • LOH (Loss of Heterozygosity) 1p/19q in Gliomas
  • Loss of Heterozygosity (LOH) 1p/19q in Gliomas
1p19q Deletion by FISH and IDH1 R132H Point Mutation by Immunohistochemistry with Reflex to IDH1 and IDH2 Mutation Analysis, Exon 4