Ordering Recommendation

Preferred initial test for the diagnosis of oligodendrogliomas. Detect IDH1 or IDH2 mutation and 1p/19q co-deletion, which are both necessary for a firm diagnosis of oligodendrogliomas.

Mnemonic
OLIGO PAN
Methodology

Fluorescence in situ Hybridization/Immunohistochemistry/Polymerase Chain Reaction/Sequencing

Performed

Mon-Fri

Reported

1-7 days, add 8-14 days if reflexed

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Tumor tissue.

Specimen Preparation

Formalin fix (10 percent neutral buffered formalin is preferred) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or 10 unstained (5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800)522-2787. (Min. 6 slides) If sending precut slides, do not oven bake.

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Paraffin block with less than 25 percent tumor tissue. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.

Remarks

Include surgical pathology report.

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Reference Interval
Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note

This test code includes pathologist interpretation.

Negative IDH1 IHC results will reflex to IDH1 and IDH2 Mutation Analysis, Exon 4, to assess for less common IDH mutations. Additional charges apply.

Hotline History
N/A
CPT Codes

88342, 88377 x2, if reflexed add 88381; 81120; 81121

Components
Component Test Code* Component Chart Name LOINC
2002148 Block ID 57723-9
3002136 IDH1 R132H Point Mut by IHC with Reflex
3003120 IDH1 Tissue Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 1p,1q codeletion FISH
  • 1p/19q Deletion
  • 1p/19q Deletion in Gliomas, FISH, Tissue
  • 1p/19q deletion in Oligodendroglioma
  • 1p/19q FISH
  • 1p/19q in oligodendrogliomas
  • 1q deletion FISH
  • Astrocytoma and Oligodendroglioma Tumor Cell Marker
  • Chromosome Analysis by FISH for 1p/19q Deletion in Gliomas
  • FISH for 1p/19q Deletion in Gliomas
  • IDH1 Point mutation IHC
  • IDH1 R132H antibody staining
  • IDH1 R132H IHC, Isocitrate dehydrogenase, 1p deletion FISH
  • LOH (Loss of Heterozygosity) 1p/19q in Gliomas
  • Loss of Heterozygosity (LOH) 1p/19q in Gliomas
1p19q Deletion by FISH and IDH1 R132H Point Mutation by Immunohistochemistry with Reflex to IDH1 and IDH2 Mutation Analysis, Exon 4