Ordering Recommendation

May be useful for evaluation of interstitial lung disease in the context of systemic autoimmune rheumatic disease (ie, connective tissue disease).

Mnemonic

ILD PANEL

Methodology

Qualitative Immunoprecipitation/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Quantitative Immunoturbidimetry

Performed

Sun-Sat

Reported

7-18 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer five 1 mL serum aliquots to ARUP Standard Transport Tubes. (Min: 0.5 mL/aliquot)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated or contaminated specimens.

Remarks
Stability

Ambient: 24 hours; Refrigerated: 1 weeks; Frozen: 1 month

Reference Interval

Test Number
Components
Reference Interval
  Rheumatoid Factor 0-14 IU/mL
  Antinuclear Antibody (ANA), HEp-2, IgG Less than 1:80
  Scleroderma (Scl-70) (ENA) Antibody, IgG 40 AU/mL or less
  SSA-52 (Ro52) (ENA) Antibody, IgG 40 AU/mL or less
  Cyclic Citrullinated Peptide Ab, IgG 0-19 Units
  Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 40 AU/mL or less

  PL-7 (threonyl-tRNA synthetase) Antibody Negative
  PL-12 (alanyl-tRNA synthetase) Antibody Negative
  EJ (glycyl-tRNA synthetase) Antibody Negative
  Ku Antibody Negative
  SRP (Signal Recognition Particle) Ab Negative
  OJ (isoleucyl-tRNA synthetase) Antibody Negative
  SSA-60 (Ro60) (ENA) Antibody, IgG 40 AU/mL or less
  MDA5 (CADM-140) Ab Negative
  NXP2 (Nuclear matrix protein-2) Ab Negative
  PM/Scl 100 Antibody, IgG Negative
  RNA Polymerase III Antibody, IgG 19 Units or less

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.


Component Interpretation
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less
30-40 AU/mL
41 AU/mL or greater
Negative
Equivocal
Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less
30-40 AU/mL
41 AU/mL or greater
Negative
Equivocal
Positive
Scleroderma (Scl-70) (ENA) Antibody, IgG 29 AU/mL or less
30-40 AU/mL
41 AU/mL or greater
Negative
Equivocal
Positive
Jo-1 Antibody, IgG 29 AU/mL or less
30-40 AU/mL
41 AU/mL or greater
Negative
Equivocal
Positive
Cyclic Citrullinated Peptide (CCP) Antibody, IgG 19 Units or less
20-39 Units
40-59 Units
60 Units or Greater
Negative
Weak positive
Moderate positive
Strong positive
RNA Polymerase III Antibody, IgG 19 Units or less
20-39 Units
40-80 Units
81 Units or greater
Negative
Weak positive
Moderate positive
Strong positive

Compliance Category

Laboratory Developed Test (LDT)

Note

Antibodies: Ro52, Ro60, Jo-1, PL-7, PL12, EJ, Ku, SRP, OJ, PM/Scl-100, MDA5, CCP, Scl-70, RA, ANA, NXP-2, RNA Polymerase III

Hotline History

N/A

CPT Codes

86235 x5; 83516 x7; 84182 x2; 86431; 86200; 86039

Components

Component Test Code* Component Chart Name LOINC
0050465 Rheumatoid Factor 11572-5
0050599 Scleroderma (Scl-70) (ENA) Antibody, IgG 47322-3
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
0055256 Cyclic Citrullinated Peptide Ab, IgG 33935-8
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
2001602 RNA Polymerase III Antibody, IgG 79182-2
2003041 PM/Scl 100 Antibody, IgG 81732-0
2010853 PL-7 (threonyl-tRNA synthetase) Antibody 33772-5
2010854 PL-12 (alanyl-tRNA synthetase) Antibody 33771-7
2010856 EJ (glycyl-tRNA synthetase) Antibody 45149-2
2010857 Ku Antibody 18484-6
2010859 SRP (Signal Recognition Particle) Ab 33921-8
2010860 OJ (isoleucyl-tRNA synthetase) Antibody 45152-6
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
2013998 Interpretive Information 48767-8
3000090 Antinuclear Antibody (ANA), HEp-2, IgG 21424-7
3000096 ANA Interpretive Comment 49311-4
3001787 MDA5 (CADM-140) Ab 82424-3
3001788 NXP2 (Nuclear matrix protein-2) Ab 82426-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

Interstitial Lung Disease Autoantibody Panel