Ordering Recommendation

May be useful for evaluation of interstitial lung disease in the context of systemic autoimmune rheumatic disease (ie, connective tissue disease).

Mnemonic
ILD PANEL
Methodology

Qualitative Immunoprecipitation/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Quantitative Immunoturbidimetry

Performed

Sun-Sat

Reported

7-18 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer five 1 mL serum aliquots to ARUP Standard Transport Tubes. (Min: 0.5 mL/aliquot)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated or contaminated specimens.

Remarks
Stability

Ambient: 24 hours; Refrigerated: 1 weeks; Frozen: 1 month

Reference Interval
Test Number
Components
Reference Interval
2012074 SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
Test Number
Components
Reference Interval
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

0050599 Scleroderma (Scl-70) (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

0099592 Jo-1 Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

2003040 PM/Scl-100 Antibody, IgG by Immunoblot Negative
0050465 Rheumatoid Factor 0-14 IU/mL
0055256 Cyclic Citrullinated Peptide (CCP) Antibody, IgG
19 Units or less Negative
20-39 Units Weak positive
40-59 Units Moderate positive
60 Units or Greater Strong positive

3000082 Antinuclear Antibody (ANA) with HEp-2 Substrate, IgG by IFA Less than 1:80
2001601 RNA Polymerase III Antibody, IgG
19 Units or less Negative
20-39 Units Weak Positive
40-80 Units Moderate Positive
81 Units or greater Strong Positive

  PL-7 (threonyl-tRNA synthetase) Antibody Negative
  PL-12 (alanyl-tRNA synthetase) Antibody Negative
  EJ (glycyl-tRNA synthetase) Antibody Negative
  Ku Antibody Negative
  SRP (Signal Recognition Particle) Ab Negative
  OJ (isoleucyl-tRNA synthetase) Antibody Negative
  NXP2 (Nuclear matrix protein-2) Ab Negative
  MDA5 (CADM-140) Ab Negative

Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note

Antibodies: Ro52, Ro60, Jo-1, PL-7, PL12, EJ, Ku, SRP, OJ, PM/Scl-100, MDA5, CCP, Scl-70, RA, ANA, NXP-2, RNA Polymerase III

Hotline History
N/A
CPT Codes

83516 x7; 86235 x5; 84182 x2; 86200; 86431; 86039

Components
Component Test Code* Component Chart Name LOINC
0050465 Rheumatoid Factor 11572-5
0050599 Scleroderma (Scl-70) (ENA) Antibody, IgG 47322-3
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
0055256 Cyclic Citrullinated Peptide Ab, IgG 33935-8
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
2001602 RNA Polymerase III Antibody, IgG 79182-2
2003041 PM/Scl 100 Antibody, IgG 81732-0
2010853 PL-7 (threonyl-tRNA synthetase) Antibody 33772-5
2010854 PL-12 (alanyl-tRNA synthetase) Antibody 33771-7
2010856 EJ (glycyl-tRNA synthetase) Antibody 45149-2
2010857 Ku Antibody 18484-6
2010859 SRP (Signal Recognition Particle) Ab 33921-8
2010860 OJ (isoleucyl-tRNA synthetase) Antibody 45152-6
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
2013998 Interpretive Information 48767-8
3000090 Antinuclear Antibody (ANA), HEp-2, IgG 21424-7
3000096 ANA Interpretive Comment 49311-4
3001787 MDA5 (CADM-140) Ab 82424-3
3001788 NXP2 (Nuclear matrix protein-2) Ab 82426-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
Interstitial Lung Disease Autoantibody Panel