Ordering Recommendation

May be useful for evaluation of interstitial lung disease in the context of systemic autoimmune rheumatic disease (ie, connective tissue disease).

Methodology

Qualitative Immunoprecipitation/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Quantitative Immunoturbidimetry

Performed

Sun-Sat

Reported

7-18 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer five 1 mL serum aliquots to ARUP Standard Transport Tubes. (Min: 2.8 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated or contaminated specimens.

Remarks
Stability

Ambient: 24 hours; Refrigerated: 1 weeks; Frozen: 1 month

Reference Interval

Test Number
Components
Reference Interval
  Rheumatoid Factor 0-14 IU/mL
  Antinuclear Antibody (ANA), HEp-2, IgG Less than 1:80
  Scleroderma (Scl-70) (ENA) Antibody, IgG 40 AU/mL or less
  SSA-52 (Ro52) (ENA) Antibody, IgG 40 AU/mL or less
  Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 40 AU/mL or less
  PL-7 (threonyl-tRNA synthetase) Antibody Negative
  PL-12 (alanyl-tRNA synthetase) Antibody Negative
  EJ (glycyl-tRNA synthetase) Antibody Negative
  Ku Antibody Negative
  SRP (Signal Recognition Particle) Ab Negative
  OJ (isoleucyl-tRNA synthetase) Antibody Negative
  SSA-60 (Ro60) (ENA) Antibody, IgG 40 AU/mL or less
  MDA5 (CADM-140) Ab Negative
  NXP2 (Nuclear matrix protein-2) Ab Negative
  PM/Scl 100 Antibody, IgG Negative
  RNA Polymerase III Antibody, IgG 19 Units or less
  Cyclic Citrullinated Peptide Ab, IgG/A 19 Units or less

Interpretive Data

Refer to report.


Component
Interpretation
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less
30-40 AU/mL
41 AU/mL or greater
Negative
Equivocal
Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less
30-40 AU/mL
41 AU/mL or greater
Negative
Equivocal
Positive
Scleroderma (Scl-70) (ENA) Antibody, IgG 29 AU/mL or less
30-40 AU/mL
41 AU/mL or greater
Negative
Equivocal
Positive
Jo-1 Antibody, IgG 29 AU/mL or less
30-40 AU/mL
41 AU/mL or greater
Negative
Equivocal
Positive
Cyclic Citrullinated Peptide (CCP) Ab, IgG/A 19 Units or less
20-39 Units
40-59 Units
60 Units or Greater
Negative
Weak positive
Moderate positive
Strong positive
RNA Polymerase III Antibody, IgG 19 Units or less
20-39 Units
40-80 Units
81 Units or greater
Negative
Weak positive
Moderate positive
Strong positive

Compliance Category

Laboratory Developed Test (LDT)

Note

Antibodies: Ro52, Ro60, Jo-1, PL-7, PL12, EJ, Ku, SRP, OJ, PM/Scl-100, MDA5, CCP, Scl-70, RA, ANA, NXP-2, RNA Polymerase III

Hotline History

N/A

CPT Codes

86235 x5; 83516 x7; 84182 x2; 86431; 86200; 86039

Components

Component Test Code* Component Chart Name LOINC
0050465 Rheumatoid Factor 11572-5
0050599 Scleroderma (Scl-70) (ENA) Antibody, IgG 47322-3
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
2001602 RNA Polymerase III Antibody, IgG 79182-2
2003041 PM/Scl 100 Antibody, IgG 81732-0
2010853 PL-7 (threonyl-tRNA synthetase) Antibody 33772-5
2010854 PL-12 (alanyl-tRNA synthetase) Antibody 33771-7
2010856 EJ (glycyl-tRNA synthetase) Antibody 45149-2
2010857 Ku Antibody 18484-6
2010859 SRP (Signal Recognition Particle) Ab 33921-8
2010860 OJ (isoleucyl-tRNA synthetase) Antibody 45152-6
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
2013998 Interpretive Information 48767-8
3000090 Antinuclear Antibody (ANA), HEp-2, IgG 21424-7
3000096 ANA Interpretive Comment 49311-4
3001787 MDA5 (CADM-140) Ab 82424-3
3001788 NXP2 (Nuclear matrix protein-2) Ab 82426-8
3016633 Cyclic Citrullinated Peptide Ab, IgG/A 57093-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

Interstitial Lung Disease Autoantibody Panel