Ordering Recommendation

May be useful for differential evaluation of polymyositis, dermatomyositis, necrotizing autoimmune myopathy, or overlap syndromes associated with connective tissue disease.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube (SST), red top tube

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer three 1 mL serum aliquots to ARUP Standard Transport Tubes. (Min: 0.5 mL/aliquot)

Storage/Transport Temperature


Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated or contaminated specimens.


Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 month


Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoprecipitation/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot




7-18 days

Reference Interval

Test Number
Reference Interval
  Smith/RNP (ENA) Ab, IgG 19 Units or less
  SSA-52 (Ro52) (ENA) Antibody, IgG 40 AU/mL or less
  Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 40 AU/mL or less
  PM/Scl 100 Antibody, IgG Negative
  Mi-2 (nuclear helicase protein) Antibody Negative
  PL-7 (threonyl-tRNA synthetase) Antibody Negative
  PL-12 (alanyl-tRNA synthetase) Antibody Negative
  P155/140 Antibody Negative
  EJ (glycyl-tRNA synthetase) Antibody Negative
  Ku Antibody Negative
  SRP (Signal Recognition Particle) Ab Negative
  OJ (isoleucyl-tRNA synthetase) Antibody Negative
  SSA-60 (Ro60) (ENA) Antibody, IgG 40 AU/mL or less
  Fibrillarin (U3 RNP) Ab, IgG Negative
  SAE1 (SUMO activating enzyme) Ab Negative
  MDA5 (CADM-140) Ab Negative
  NXP2 (Nuclear matrix protein-2) Ab Negative
  TIF-1 gamma (155 kDa) Ab Negative

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
Smith/RNP (ENA) Antibody, IgG 19 Units or less  Negative
20-39 Units  Weak Positive
40-80 Units  Moderate Positive
81 Units or greater  Strong Positive
Jo-1 Antibody, IgG 29 AU/mL or less  Negative
30-40 AU/mL  Equivocal
41 AU/mL or greater  Positive

Compliance Category

Laboratory Developed Test (LDT)


Antibodies: Mi-2, PL-7, PL12, P155/140, EJ, Ku, OJ, PM/Scl, SRP, Smith/RNP, Ro52, Ro60, Jo-1, U3 Fib, SAE1, NXP2, MDA5, TIF1-gamma

Hotline History


CPT Codes

83516 x8; 86235 x6; 84182 x4


Component Test Code* Component Chart Name LOINC
0050470 Smith/RNP (ENA) Ab, IgG 33983-8
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
2003041 PM/Scl 100 Antibody, IgG 81732-0
2010852 Mi-2 (nuclear helicase protein) Antibody 18485-3
2010853 PL-7 (threonyl-tRNA synthetase) Antibody 33772-5
2010854 PL-12 (alanyl-tRNA synthetase) Antibody 33771-7
2010855 P155/140 Antibody 8061-4
2010856 EJ (glycyl-tRNA synthetase) Antibody 45149-2
2010857 Ku Antibody 18484-6
2010859 SRP (Signal Recognition Particle) Ab 33921-8
2010860 OJ (isoleucyl-tRNA synthetase) Antibody 45152-6
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
2012174 Fibrillarin (U3 RNP) Ab, IgG 49963-2
2013994 Myositis Panel Interpretive Data 48767-8
3001786 SAE1 (SUMO activating enzyme) Ab 82440-9
3001787 MDA5 (CADM-140) Ab 82424-3
3001788 NXP2 (Nuclear matrix protein-2) Ab 82426-8
3001789 TIF-1 gamma (155 kDa) Ab 82448-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Extended Myositis Panel