Ordering Recommendation

May be useful for differential evaluation of polymyositis, dermatomyositis, necrotizing autoimmune myopathy, or overlap syndromes associated with connective tissue disease.

Mnemonic
MYOS EXT
Methodology

Qualitative Immunoprecipitation/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot

Performed

Sun-Sat

Reported

7-18 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer three 1 mL serum aliquots to ARUP Standard Transport Tubes. (Min: 0.5 mL/aliquot)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Hemolyzed, hyperlipemic, icteric, heat-treated or contaminated specimens.

Remarks
Stability

Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Reference Interval
Test Number
Components
Reference Interval
2012074 SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
Test Number
Components
Reference Interval
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

0050470 Smith/RNP (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

0099592 Jo-1 Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

2012173 Fibrillarin (U3 RNP) Antibody, IgG Negative
2003040 PM/Scl-100 Antibody, IgG by Immunoblot Negative
  Mi-2 (nuclear helicase protein) Antibody Negative
  PL-7 (threonyl-tRNA synthetase) Antibody Negative
  PL-12 (alanyl-tRNA synthetase) Antibody Negative
  P155/140 Antibody Negative
  EJ (glycyl-tRNA synthetase) Antibody Negative
  Ku Antibody Negative
  SRP (Signal Recognition Particle) Ab Negative
  OJ (isoleucyl-tRNA synthetase) Antibody Negative
  SAE1 (SUMO activating enzyme) Ab Negative
  MDA5 (CADM-140) Ab Negative
  NXP2 (Nuclear matrix protein-2) Ab Negative
  TIF-1 gamma (155 kDa) Ab Negative

Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note

Antibodies: Mi-2, PL-7, PL12, P155/140, EJ, Ku, OJ, PM/Scl, SRP, Smith/RNP, Ro52, Ro60, Jo-1, U3 Fib, SAE1, NXP2, MDA5, TIF1-gamma

Hotline History
N/A
CPT Codes

83516 x8; 86235 x6; 84182 x4

Components
Component Test Code* Component Chart Name LOINC
0050470 Smith/RNP (ENA) Ab, IgG 33983-8
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
0099592 Jo-1 (Histidyl-tRNA Synthetase) Ab, IgG 33571-1
2003041 PM/Scl 100 Antibody, IgG 81732-0
2010852 Mi-2 (nuclear helicase protein) Antibody 18485-3
2010853 PL-7 (threonyl-tRNA synthetase) Antibody 33772-5
2010854 PL-12 (alanyl-tRNA synthetase) Antibody 33771-7
2010855 P155/140 Antibody
2010856 EJ (glycyl-tRNA synthetase) Antibody 45149-2
2010857 Ku Antibody 18484-6
2010859 SRP (Signal Recognition Particle) Ab 33921-8
2010860 OJ (isoleucyl-tRNA synthetase) Antibody 45152-6
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
2012174 Fibrillarin (U3 RNP) Ab, IgG 49963-2
2013994 Myositis Panel Interpretive Data 48767-8
3001786 SAE1 (SUMO activating enzyme) Ab 82440-9
3001787 MDA5 (CADM-140) Ab 82424-3
3001788 NXP2 (Nuclear matrix protein-2) Ab 82426-8
3001789 TIF-1 gamma (155 kDa) Ab 82448-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
Extended Myositis Panel