Ordering Recommendation

Aid in the diagnosis of aggressive large B-cell lymphoma with intermediate features between Burkitt lymphoma and diffuse large B-cell lymphoma (DLBCL) and confirmation of suspected double-hit lymphoma.

Mnemonic
DLBCL_RFLX
Methodology

Fluorescence in situ Hybridization (FISH)

Performed

Varies

Reported

3-7 days, if reflexed, add 3 days for each reflex

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Tumor tissue.

Specimen Preparation

Formalin fix (10 percent neutral buffered formalin) and paraffin-embed tissue. Transport tissue block or 8 unstained 3-micron slides. (Min: 4 slides) Protect paraffin block from excessive heat. Transport block(s) and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.

Remarks

Include surgical pathology report.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Reference Interval
Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Analyte Specific Reagent (ASR)

Note

If Aggressive B-Cell Lymphoma Reflex Panel by FISH is positive, then IGH-BCL2 Fusion, t(14;18) by FISH (ARUP test code 3001298) will be added. If IGH-BCL2 Fusion, t(14;18) by FISH is negative, then BCL6 (3q27) Gene Rearrangement by FISH (ARUP test code 3001311) will be added. Additional charges apply.

Hotline History
N/A
CPT Codes

88366, if reflexed add 88366; if further reflexed add 88366

Components
Component Test Code* Component Chart Name LOINC
3000616 MYC FISH Reference Number
3000617 MYC FISH Source
3000618 MYC FISH Result
3000702 Total Cell Count 78236-7
3000703 Scoring Method 81304-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 8q24 gene rearrangement FISH
  • Burkitt's Lymphoma; DLBCL
  • Double hit lymphoma, BCL6 FISH, BCL2 FISH
  • LSI MYC FISH
  • MYC FISH
  • MYC gene rearrangement FISH
Aggressive B-Cell Lymphoma Reflex Panel by FISH, Tissue