Ordering Recommendation

Aid in the diagnosis of aggressive large B-cell lymphoma with intermediate features between Burkitt lymphoma and diffuse large B-cell lymphoma (DLBCL) and confirmation of suspected double-hit lymphoma.


Fluorescence in situ Hybridization (FISH)




3-7 days, if reflexed, add 3 days for each reflex

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Tumor tissue.

Specimen Preparation

Formalin fix (10 percent neutral buffered formalin) and paraffin-embed tissue. Transport tissue block or 8 unstained 3-micron slides. (Min: 4 slides) Protect paraffin block from excessive heat. Transport block(s) and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.


Include surgical pathology report.


Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Reference Interval
Interpretive Data

Refer to report.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.


If Aggressive B-Cell Lymphoma Reflex Panel by FISH is positive, then IGH-BCL2 Fusion, t(14;18) by FISH (ARUP test code 3001298) will be added. If IGH-BCL2 Fusion, t(14;18) by FISH is negative, then BCL6 (3q27) Gene Rearrangement by FISH (ARUP test code 3001311) will be added. Additional charges apply.

Hotline History
CPT Codes

88366, if reflexed add 88366; if further reflexed add 88366

Component Test Code* Component Chart Name LOINC
3000616 MYC FISH Reference Number
3000617 MYC FISH Source
3000618 MYC FISH Result
3000702 Total Cell Count 78236-7
3000703 Scoring Method 81304-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • 8q24 gene rearrangement FISH
  • Burkitt's Lymphoma; DLBCL
  • Double hit lymphoma, BCL6 FISH, BCL2 FISH
  • MYC gene rearrangement FISH
Aggressive B-Cell Lymphoma Reflex Panel by FISH, Tissue