Lymphocyte Proliferation, Anti-CD3, Anti-CD28 and IL-2 Induced, by Flow Cytometry (24-Hr Critical Room Temp)
Primarily for evaluating T-cell function in patients with suspected cellular immune dysfunction, such as primary and secondary immunodeficiencies. Other uses include monitoring lymphocyte recovery and competence after hematopoietic stem cell transplantation and monitoring lymphocyte function during immunosuppressive therapy. Not a first-level test; order after Lymphocyte Proliferation, Mitogen Induced, by Flow Cytometry (48-Hr Critical Room Temp) (3001321) has been performed.
Cell Culture/Flow Cytometry
Collect control specimen from a healthy individual unrelated to patient.
Patient and control specimens must be collected ONLY on Wednesdays or Thursdays AND shipped directly to ARUP the same calendar day to meet the strict 24-hour stability requirement.
Green (sodium heparin) (patient) AND green (sodium heparin) (control) only on Wednesdays or Thursdays.
: Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)). Infant Minimum: 3 mL (patient) AND 7 mL (control).
Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
CRITICAL ROOM TEMPERATURE.
Must be collected and shipped directly to ARUP the same calendar day.
Refrigerated or frozen specimens.
Do not collect or ship on, or the day before, holidays.
Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
|CD45 Pos Cells||10.2% or greater||29.0% or greater||33.1% or greater|
|CD3 Pos Cells||13.5% or greater||24.3% or greater||36.1% or greater|
This test measures T lymphocyte proliferation in response to stimulation with anti-CD3, anti-CD3/anti-CD28, and anti-CD3/Interleukin-2, determined by flow cytometry. Proliferating cells are detected by fluorescent labeling. Results are reported as percent proliferating cells of the total specific cell populations. This is a second-level test to be performed after Lymphocyte Proliferation to Mitogens (PHA, Con A, and PWM) by Flow Cytometry has been assessed.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|3001338||CD45+ Cell Proliferation by anti-CD3||81760-1|
|3001339||CD3+ Cell Proliferation by anti-CD3||81756-9|
|3001340||CD45+ Cell Prolif by anti-CD3/CD28||81759-3|
|3001341||CD3+ Cell Prolif by anti-CD3/CD28||81758-5|
|3001342||CD45+ Cell Prolif by anti-CD3 and IL-2||81755-1|
|3001343||CD3+ Cell Prolif by anti-CD3 and IL-2||81757-7|
|3001344||Lymphocyte Proliferation-AntiCD3, Interp||53810-8|