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Time Sensitive

Ordering Recommendation

Primarily for evaluating T-cell function in patients with suspected cellular immune dysfunction, such as primary and secondary immunodeficiencies. Other uses include monitoring lymphocyte recovery and competence after hematopoietic stem cell transplantation and monitoring lymphocyte function during immunosuppressive therapy. Not a first-level test; order after Lymphocyte Proliferation, Mitogen Induced, by Flow Cytometry (48-Hr Critical Room Temp) (3001321) has been performed.

Mnemonic

LPCD3 FLOW

Methodology

Cell Culture/Flow Cytometry

Performed

Thu, Fri

Reported

9-10 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Collect control specimen from a healthy individual unrelated to patient.
Patient and control specimens must be collected ONLY on Wednesdays or Thursdays AND shipped directly to ARUP the same calendar day to meet the strict 24-hour stability requirement.

Collect

Green (sodium heparin) (patient) AND green (sodium heparin) (control) only on Wednesdays or Thursdays.

Specimen Preparation

: Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)). Infant Minimum: 3 mL (patient) AND 7 mL (control).
Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.

Storage/Transport Temperature

CRITICAL ROOM TEMPERATURE.
Must be collected and shipped directly to ARUP the same calendar day.

Unacceptable Conditions

Refrigerated or frozen specimens.

Remarks

Do not collect or ship on, or the day before, holidays.

Stability

Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

Anti-CD3 Anti-CD3/Anti-CD28 Anti-CD3/IL-2
CD45 Pos Cells 10.2% or greater 29.0% or greater 33.1% or greater
CD3 Pos Cells 13.5% or greater 24.3% or greater 36.1% or greater

Interpretive Data

This test measures T lymphocyte proliferation in response to stimulation with anti-CD3, anti-CD3/anti-CD28, and anti-CD3/Interleukin-2, determined by flow cytometry. Proliferating cells are detected by fluorescent labeling. Results are reported as percent proliferating cells of the total specific cell populations. This is a second-level test to be performed after Lymphocyte Proliferation to Mitogens (PHA, Con A, and PWM) by Flow Cytometry has been assessed.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

86353 x3

Components

Component Test Code* Component Chart Name LOINC
3001338 CD45+ Cell Proliferation by anti-CD3 81760-1
3001339 CD3+ Cell Proliferation by anti-CD3 81756-9
3001340 CD45+ Cell Prolif by anti-CD3/CD28 81759-3
3001341 CD3+ Cell Prolif by anti-CD3/CD28 81758-5
3001342 CD45+ Cell Prolif by anti-CD3 and IL-2 81755-1
3001343 CD3+ Cell Prolif by anti-CD3 and IL-2 81757-7
3001344 Lymphocyte Proliferation-AntiCD3, Interp 53810-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

Lymphocyte Proliferation, Anti-CD3, Anti-CD28 and IL-2 Induced, by Flow Cytometry (24-Hr Critical Room Temp)