Ordering Recommendation

Aid in prognostication and therapeutic decisions for neoplasms where amplification has been demonstrated. Not recommended for EGFR mutation detection in non-small cell lung carcinoma (NSCLC); preferred test is EGFR Mutation Detection by Pyrosequencing (2002440).


Fluorescence in situ Hybridization (FISH)




3-7 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Tumor tissue.

Specimen Preparation

Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect paraffin block from excessive heat. Transport tissue block or 5 unstained (4-micron thick sections) positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787 (kit recommended but not necessary). (Min 2 slides)

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.


Include surgical pathology report


Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Refer to report.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

Hotline History
CPT Codes


Component Test Code* Component Chart Name LOINC
3000674 EGFR FISH Reference Number
3000675 EGFR FISH Source
3000676 EGFR/CEP7 FISH Ratio
3000677 Average EGFR Signal Number per Cell
3000678 Average CEP7 Signal Number per Cell
3000679 EGFR FISH Result
3000703 Scoring Method 81304-8
3002939 Total Cell Count 78236-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • EGFR amplification by FISH
  • EGFR; EGFR (Her-1)
  • Epidermal Growth Factor Receptor
  • erbB-1
  • Her-1
EGFR Gene Amplification by FISH