Aid in prognostication and therapeutic decisions for neoplasms where amplification has been demonstrated. Not recommended for EGFR mutation detection in non-small cell lung carcinoma (NSCLC); preferred test is EGFR Mutation Detection by Pyrosequencing (2002440).
Fluorescence in situ Hybridization (FISH)
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect paraffin block from excessive heat. Transport tissue block or 5 unstained (4-micron thick sections) positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787 (kit recommended but not necessary). (Min 2 slides)
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.
Include surgical pathology report
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Refer to report.
Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.
|Component Test Code*||Component Chart Name||LOINC|
|3000674||EGFR FISH Reference Number|
|3000675||EGFR FISH Source|
|3000676||EGFR/CEP7 FISH Ratio|
|3000677||Average EGFR Signal Number per Cell|
|3000678||Average CEP7 Signal Number per Cell|
|3000679||EGFR FISH Result|
|3000702||Total Cell Count||78236-7|
- EGFR amplification by FISH
- EGFR FISH
- EGFR; EGFR (Her-1)
- Epidermal Growth Factor Receptor