Ordering Recommendation

Detect ROS1 gene rearrangements in solid tumors. Does not identify the translocation partner or variant.

Mnemonic
ROS1_FISH
Methodology

Fluorescence in situ Hybridization (FISH)

Performed

Monday-Friday

Reported

3-7 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Tumor tissue.

Specimen Preparation

Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Protect paraffin block from excessive heat. Transport tissue block or 5 unstained (4- micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808), available online through eSupply using ARUP Connector contact ARUP Client Services at (800) 522-2787 (kit recommended but not necessary). (Min: 2 slides)

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.

Remarks

Include surgical pathology report.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Reference Interval
Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

88366

Components
Component Test Code* Component Chart Name LOINC
3000654 ROS1 FISH Reference Number
3000655 ROS1 FISH Source
3000656 ROS1 FISH Result 59050-5
3000703 Scoring Method 81304-8
3002951 Total Cell Count 78236-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
ROS1 by FISH