Most sensitive method to detect IGH-BCL2 fusion in formalin-fixed, paraffin-embedded (FFPE) tissue specimens.
Fluorescence in situ Hybridization (FISH)
New York DOH Approval Status
Formalin fix (10 percent neutral buffered formalin) and paraffin-embed specimen. Protect paraffin block from excessive heat. Transport tissue block or 5 unstained (3-micron thick sections) positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787 (kit is recommended by not necessary). (Min: 2 slides)
Room temperature or refrigerated. Ship in cooled container during summer months.
Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.
Include surgical pathology report.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Refer to report.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Analyte Specific Reagent (ASR)
|Component Test Code*||Component Chart Name||LOINC|
|3000608||BCL2 FISH Reference Number||89873-4|
|3000609||BCL2 FISH Source||31208-2|
|3000610||BCL2 FISH Result||77038-8|
|3002936||Total Cell Count||78236-7|
- IGH-BCL2 Fusion FISH
- IGH-BCL2 rearrangement FISH
- t(14;18) FISH