Ordering Recommendation

Use in conjunction with histologic and clinical information for the diagnosis of alveolar rhabdomyosarcoma.

Mnemonic
FKHR_FISH
Methodology

Fluorescence in situ Hybridization (FISH)

Performed

Monday-Friday

Reported

3-7 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect paraffin block from excessive heat.  Transport tissue block or 5 unstained (4-micron thick sections) positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787 (kit recommended, but not necessary). (Min. 2 slides)

Collect

Tumor tissue.

Specimen Preparation
Storage/Transport Temperature

Room temperature or refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.

Remarks

Include surgical pathology report.

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Refer to report.

Compliance Category

Analyte Specific Reagent (ASR)

Note
Hotline History
N/A
CPT Codes

88366

Components
Component Test Code* Component Chart Name LOINC
3000604 FOXO1 FISH Reference Number
3000605 FOXO1 FISH Source
3000606 FOXO1 FISH Result
3000703 Scoring Method 81304-8
3002941 Total Cell Count 78236-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 13q14
  • 13q14 (FOX01 or FKHR) Rearrangement FISH
  • Alveolar Rhabdomyosarcoma
  • FKHR FISH
  • FKHR gene break-apart FISH
  • FOX01 FISH
  • FOX01 gene break-apart FIS
  • FOXO1 (13q14) rearrangements FISH
  • PAX7/FOXO1A
FOXO1 (FKHR) (13q14) Gene Rearrangement by FISH