Ordering Recommendation

Use in conjunction with histologic and clinical information for the diagnosis of alveolar rhabdomyosarcoma.

Mnemonic

FKHR_FISH

Methodology

Fluorescence in situ Hybridization (FISH)

Performed

Monday-Friday

Reported

3-7 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect paraffin block from excessive heat.  Transport tissue block or 5 unstained (4-micron thick sections) positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787 (kit recommended, but not necessary). (Min. 2 slides)

Collect

Tumor tissue.

Specimen Preparation
Storage/Transport Temperature

Room temperature or refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Specimens fixed or processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). No tumor in tissue. Decalcified specimens.

Remarks

Include surgical pathology report.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Analyte Specific Reagent (ASR)

Note

Hotline History

N/A

CPT Codes

88366

Components

Component Test Code* Component Chart Name LOINC
3000604 FOXO1 FISH Reference Number
3000605 FOXO1 FISH Source
3000606 FOXO1 FISH Result
3000703 Scoring Method 81304-8
3002941 Total Cell Count 78236-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • 13q14
  • 13q14 (FOX01 or FKHR) Rearrangement FISH
  • Alveolar Rhabdomyosarcoma
  • FKHR FISH
  • FKHR gene break-apart FISH
  • FOX01 FISH
  • FOX01 gene break-apart FIS
  • FOXO1 (13q14) rearrangements FISH
  • PAX7/FOXO1A
FOXO1 (FKHR) (13q14) Gene Rearrangement by FISH