Ordering Recommendation

Aids in the diagnosis and management of acute myeloid leukemia. Not intended for minimal residual disease monitoring.

Mnemonic
FLT3-PCR
Methodology

Polymerase Chain Reaction

Performed

DNA isolation: Sun-Sat
Assay:
Mon-Sat

Reported

2-7 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA) or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA certified lab.

Specimen Preparation

Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow:
Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.

Remarks
Stability

Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely

Reference Interval
Interpretive Data

Refer to report. 

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

81245; 81246

Components
Component Test Code* Component Chart Name LOINC
3001162 FLT3 Source 31208-2
3001163 ITD Result 79210-1
3001164 ITD Ratio 92844-0
3001165 TKD Result 69548-6
3001166 FLT3 ITD and TKD Mutation Detection 50398-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • FLT3 CDX
  • FLT3 D835
  • FLT3 ITD
  • FLT3 Mutant
  • FLT3 Ratio
  • FLT3 Signal Ratio
  • FLT3 TKD
FLT3 ITD and TKD Mutation Detection