Ordering Recommendation

Detect Acanthamoeba spp, Naegleria spp, and other free-living amoebae.

Mnemonic
ACANT CSF
Methodology

Qualitative Culture/Microscopy/Giemsa Stain

Performed

Sun-Sat

Reported

2-10 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

CSF.

Specimen Preparation

Transfer 1 mL CSF to a sterile ARUP Standard Transport Tube (ARUP supply # 43115) available online through eSupply using ARUP Connect™ or contact Client Services at (800) 522-2787. (Min: 0.5 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Specimens in media or preservatives.

Remarks
Stability

Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval

Negative.

Interpretive Data

The stain will detect free-living amoeba such as Naegleria fowleri and Balamuthia mandrillaris.

The culture will detect free-living amoeba such as Acanthamoeba species and Naegleria fowleri but will NOT detect Balamuthia mandrillaris.

Compliance Category

Standard

Note

For all other specimen types refer to Acanthamoeba and Naegleria Culture (ARUP test code 0060245) and Amoeba Calcofluor Stain (ARUP test code 0060250). For Entamoeba histolytica detection refer to Entamoeba histolytica Antigen, EIA (ARUP test code 0058001).

Hotline History
N/A
CPT Codes

87081; 87207

Components
Component Test Code* Component Chart Name LOINC
0060245 Acanthamoeba and Naegleria Culture 9781-6
2003035 Amoeba, Giemsa Stain
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Culture for Acanthamoeba, Spinal Fluid
  • Culture for Naegleria, Spinal Fluid
  • Detection of Acanthamoeba/Naegleria Species
  • Naegleria Culture
  • Stain for Balamuthia mandrillaris
Acanthamoeba and Naegleria Culture and Stain, CSF