Ordering Recommendation

Detect and quantify HIV-1 in CSF.

Mnemonic
HIVCSF QNT
Methodology

Quantitative Transcription-Mediated Amplification

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Cerebral spinal fluid.

Specimen Preparation

Transfer 2 mL CSF to an ARUP Standard Transport Tube and freeze. (Min: 0.8 mL)

Storage/Transport Temperature

Frozen

Unacceptable Conditions

Plasma (refer to Human Immunodeficiency Virus 1 (HIV-1) by Quantitative NAAT, Plasma, ARUP test code 3000867).

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 5 days; Frozen: 1 month

Reference Interval

Not detected

Interpretive Data

Normal range for this assay is "Not Detected".
The quantitative range of this assay is 1.47-7.00 log copies/mL (30-10,000,000 copies/mL).

An interpretation of "Not Detected" does not rule out the presence of inhibitors or HIV-1 RNA concentration below the level of detection of the assay. Care should be taken in the interpretation of any single viral load determination.
The clinical significance of changes in HIV-1 RNA concentration has not been fully established; however, a change of 0.5 log copies/mL may be significant.

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note

The limit of quantification for this RNA assay is 1.47 log copies/mL (30 copies/mL). If the assay DETECTED the presence of the virus but was not able to accurately quantify the viral load, the test result will be reported as "<30 Detected".

This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used as an aid in assessing viral response to antiretroviral treatment as measured by changes in HIV-1 RNA concentration.

Hotline History
N/A
Components
Component Test Code* Component Chart Name LOINC
3000873 HIV-1 Qnt by NAAT, CSF (log copies/mL) 41497-9
3000971 HIV-1 Qnt by NAAT Interp, CSF 42917-5
3000995 HIV-1 Qnt by NAAT, CSF (copies/mL) 41498-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HIV quantitative
  • HIV-1 RNA
  • HIV-1 RNA Viral Load
  • HIV1 viral load monitoring
  • Human Immunodeficiency Virus 1
  • Nucleic Acid Amplification Test (NAAT)
Human Immunodeficiency Virus 1 (HIV-1) by Quantitative NAAT, CSF