Ordering Recommendation

Initial screening test for systemic autoimmune rheumatic diseases (ie, connective tissue diseases).

Mnemonic
ANA AB PAT
Methodology

Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot

Performed

Sun-Sat

Reported

1-8 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Non-serum specimens. Contaminated, grossly hemolyzed, heat-inactivated, severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
3000082 Antinuclear Antibody (ANA) with HEp-2 Substrate, IgG by IFA Less than 1:80
2003040 PM/Scl-100 Antibody, IgG by Immunoblot Negative
2012173 Fibrillarin (U3 RNP) Antibody, IgG Negative
0050215 Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA Effective August 20, 2012
Test Number
Components
Reference Interval
Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA None Detected.
2002693 Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) Less than 1:10

2002693 Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) Less than 1:10
2005287 Chromatin Antibody, IgG
19 Units or less Negative
20-60 Units Moderate Positive
61 Units or greater Strong Positive

2001601 RNA Polymerase III Antibody, IgG
19 Units or less Negative
20-39 Units Weak Positive
40-80 Units Moderate Positive
81 Units or greater Strong Positive

0050599 Scleroderma (Scl-70) (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

0050470 Smith/RNP (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

0050085 Smith (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

2012074 SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
Test Number
Components
Reference Interval
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

0050692 SSB (La) (ENA) Antibody, IgG
29 AU/mL or less Negative
30-40 AU/mL Equivocal
41 AU/mL or greater Positive

Interpretive Data

Refer to report.

No compliance statements are in use for this test.

Note

The Antinuclear Antibody (ANA), IgG by IFA with Reflex by IFA Pattern begins with Nuclear Antibody (ANA) by IFA, IgG. Depending on findings, one or more reflexive tests may be required. Tests added may include Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA; Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidiae luciliae); Chromatin Antibody, IgG; Smith/RNP (ENA) Antibody, IgG; Fibrillarin (U3 RNP) Antibody, IgG; Smith (ENA) Antibody, IgG; SSA 52 (Ro) (ENA) Antibody, IgG; SSA 60 (Ro) (ENA) Antibody, IgG; SSB (La) (ENA) Antibody, IgG; Scleroderma (Scl-70) (ENA) Antibody, IgG; PM/Scl-100 Antibody, IgG, by Immunoblot; and/or RNA Polymerase III Antibody, IgG. Additional charges apply.

ANA determined by indirect fluorescence assay (IFA) use HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. If positive, patterns reported include homogeneous, speckled, centromere, nucleolar, nuclear dots, or cytoplasmic. All positive results are reported with endpoint titers.

Hotline History
N/A
CPT Codes

86039; if homogenous or speckled pattern add 86235 x6, 86225, and 83516; if reflexed add 86256; If nucleolar pattern add 86235 x3 and 83516

Components
Component Test Code* Component Chart Name LOINC
3000090 Antinuclear Antibody (ANA), HEp-2, IgG 21424-7
3000096 ANA Interpretive Comment 49311-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Antinuclear Antibody
  • FANA
  • Fluorescent Antinuclear Antibody
Antinuclear Antibody (ANA) with HEp-2 Substrate, IgG by IFA with Reflex by Pattern