Antinuclear Antibody (ANA) with HEp-2 Substrate, IgG by IFA with Reflex by Pattern
Initial screening test for systemic autoimmune rheumatic diseases (ie, connective tissue diseases).
Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Multiplex Bead Assay/Qualitative Immunoblot
Serum Separator Tube (SST).
Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL)
Non-serum specimens. Contaminated, grossly hemolyzed, heat-inactivated, severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|3000082||Antinuclear Antibody (ANA) with HEp-2 Substrate, IgG by IFA||Less than 1:80|
|2003040||PM/Scl-100 Antibody, IgG by Immunoblot||Negative|
|2012173||Fibrillarin (U3 RNP) Antibody, IgG||Negative|
|0050215||Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA||Effective August 20, 2012
|2002693||Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae)||Less than 1:10|
|2005287||Chromatin Antibody, IgG||
|2001601||RNA Polymerase III Antibody, IgG||
|0050599||Scleroderma (Scl-70) (ENA) Antibody, IgG||
|0050470||Smith/RNP (ENA) Antibody, IgG||
|0050085||Smith (ENA) Antibody, IgG||
|2012074||SSA 52 and 60 (Ro) (ENA) Antibodies, IgG||
|0050692||SSB (La) (ENA) Antibody, IgG||
Refer to report.
The Antinuclear Antibody (ANA), IgG by IFA with Reflex by IFA Pattern begins with Nuclear Antibody (ANA) by IFA, IgG. Depending on findings, one or more reflexive tests may be required. Tests added may include Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA; Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidiae luciliae); Chromatin Antibody, IgG; Smith/RNP (ENA) Antibody, IgG; Fibrillarin (U3 RNP) Antibody, IgG; Smith (ENA) Antibody, IgG; SSA 52 (Ro) (ENA) Antibody, IgG; SSA 60 (Ro) (ENA) Antibody, IgG; SSB (La) (ENA) Antibody, IgG; Scleroderma (Scl-70) (ENA) Antibody, IgG; PM/Scl-100 Antibody, IgG, by Immunoblot; and/or RNA Polymerase III Antibody, IgG. Additional charges apply.
ANA identified by indirect fluorescence assay (IFA) using HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. Positive nuclear patterns reported include homogeneous, speckled, centromere, nucleolar, or nuclear dots. Positive cytoplasmic patterns reported include reticular/AMA, discrete/GW body-like, polar/golgi-like, rods and rings, or cytoplasmic speckled patterns. All positive results are reported with endpoint titers at no additional charge.
86039; if homogenous or speckled pattern add 86235 x6, 86225, and 83516; if reflexed add 86256; If nucleolar pattern add 86235 x3 and 83516
|Component Test Code*||Component Chart Name||LOINC|
|3000090||Antinuclear Antibody (ANA), HEp-2, IgG||21424-7|
|3000096||ANA Interpretive Comment||49311-4|
- Antinuclear Antibody
- Fluorescent Antinuclear Antibody