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QuantiFERON-TB Gold Plus, 1-Tube
3000400
Ordering Recommendation

Aid in the detection of latent disease among persons at increased risk for tuberculosis (TB). Positive predictive value is less in low-risk populations. May be used in persons who have received BCG vaccine.

Mnemonic
QFT-PLUS
Methodology
Cell Culture/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Time SensitiveTime Sensitive
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
QuantiFERON-TB Gold Plus 1-tube (ARUP Supply #54015) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. For collection and transport instructions refer to QuantiFERON under Special Handling at https://aruplab.com/testing/specimen/quantiferon. 
Specimen Preparation
Specimen must remain ambient for a minimum of 15 minutes after collection before being refrigerated. Transport 5 mL whole blood. (Min: 5 mL). 
Storage/Transport Temperature
Refrigerated. Must be collected and shipped directly to ARUP the same calendar day
Unacceptable Conditions
 
Remarks
Do not collect or ship on, or the day before, holidays. 
Stability
Ambient: 3 hours; Refrigerated: 48 hours; Frozen: Unacceptable 
Reference Interval
ComponentsReference Interval
QuantiFERON-TB Gold In-TubeNegative
QuantiFERON-TB1 minus NIL0.34 IU/mL or less
QuantiFERON-TB2 minus NIL0.34 IU/mL or less
QuantiFERON MITOGEN minus NILNo reference interval
QuantiFERON NILNo reference interval

Interpretive Data
Interferon gamma release is measured for specimens from each of the four collection tubes. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values, NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). The NIL value represents nonspecific reactivity produced by the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB1-NIL tube specifically detects CD4+ lymphocyte reactivity, specifically stimulated by the TB1 antigens. The TB2-NIL tube detects both CD4+ and CD8+ lymphocyte reactivity, stimulated by TB2 antigens. An overall Negative result does not completely rule out TB infection.

A false-positive result in the absence of other clinical evidence of TB infection is not uncommon. Refer to: Updated Guidelines for Using Interferon Gamma Release Assays to Detect Mycobacterium tuberculosis Infection --- United States, 2010 (<https://www.cdc.gov/mmwr/preview/mmwrhtml/rr5905a1.htm>), for more information concerning test performance in low-prevalence populations and use in occupational screening.

Note
If the stability requirements cannot be met, please refer to test 3000399, QuantiFERON-TB Gold Plus, 4-Tube.
Hotline History
View Hotline History
Components
Component Test Code*Component Chart NameLOINC
2006962QuantiFERON Mitogen minus NIL71774-4
2006963QuantiFERON NIL71776-9
3000401Quantiferon TB Gold Plus71773-6
3000402Quantiferon Plus TB1 minus NIL64084-7
3000403Quantiferon Plus TB2 minus NIL88517-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Interferon-Gamma Release Assay for Tuberculosis
  • Latent Tuberculosis Infection (LTBI)
  • QFT & M TB
  • QFT-G & M. tuberculosis
  • QFT-TB; QFT-TB Plus
  • Quantiferon Mitogen and NIL
  • QuantiFERON-TB Gold In Tube
  • QuantiFERON-TB Gold Plus & Mycobacterium tuberculosis Antibody