Ordering Recommendation

Not recommended for the diagnosis of fungal infection of the central nervous system. Includes testing by complement fixation for Aspergillus, Coccidioides, and Histoplasma and testing by immunoassay for Blastomyces. Refer to individual fungus-specific testing according to patient exposure.


Semi-Quantitative Complement Fixation/Semi-Quantitative Enzyme-Linked Immunosorbent Assay (ELISA)/Qualitative Immunodiffusion




2-6 days

New York DOH Approval Status

Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.

Specimen Required

Patient Preparation


Specimen Preparation

Transfer 2 mL CSF to an ARUP standard transport tube. (Min: 1 mL)

Storage/Transport Temperature


Unacceptable Conditions

Other body fluids. Contaminated, hemolyzed, xanthochromic, or severely lipemic specimens.


Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Test Number
Reference Interval
  Coccidioides Ab by CF, CSF Less than 1:2
  Aspergillus Antibody, CSF (CF) Less than 1:2
  Histoplasma M, CSF (CF) Less than 1:2
  Histoplasma Y, CSF (CF) Less than 1:2
  Blastomyces Antibodies EIA, CSF 0.9 IV or less

Interpretive Data

Refer to report.

Compliance Category

Modified FDA


This test detects antibodies to Aspergillus, Coccidioides, and Histoplasma by complement fixation and Blastomyces by immunoassay.
If Blastomyces antibodies are equivocal or positive by immunoassay then Blastomyces dermatitidis Antibodies by Immunodiffusion, CSF will be added. Additional charges apply.

Hotline History


CPT Codes

86606; 86612; 86635; 86698 x2; if reflexed, add 86612


Component Test Code* Component Chart Name LOINC
0050711 Coccidioides Ab by CF, CSF 13917-0
0050751 Aspergillus Antibody, CSF (CF) 23953-3
0050753 Histoplasma M, CSF (CF) 27220-3
0050754 Histoplasma Y, CSF (CF) 27209-6
3000233 Blastomyces Antibodies EIA, CSF 13201-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Aspergillus
  • Blastomyces
  • Coccidiodes
  • fungal meningitis
  • Histoplasma
Fungal Antibodies with Reflex to Blastomyces dermatitidis Antibodies by Immunodiffusion, CSF