Ordering Recommendation

Not recommended for the diagnosis of fungal infection of the central nervous system. Refer to individual fungal antibody tests, as relevant to patient exposure(s).




Semi-Quantitative Complement Fixation/Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunodiffusion




2-6 days

New York DOH Approval Status

Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.

Specimen Required

Patient Preparation


Specimen Preparation

Transfer 1 mL CSF to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature


Unacceptable Conditions

Other body fluids. Contaminated, hemolyzed, xanthochromic, or severely lipemic specimens.


Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Test Number
Reference Interval
  Aspergillus Antibody, CSF (CF) Less than 1:2
3000231 Blastomyces dermatitidis Antibodies by EIA with Reflex to Immunodiffusion, CSF
Test Number
Reference Interval
3000230 Fungal Antibodies with Reflex to Blastomyces dermatitidis Antibodies by Immunodiffusion, CSF 0.9 IV or less: Negative
1.0-1.4 IV: Equivocal
1.5 IV or greater: Positive
Blastomyces Ab by Immunodiffusion, CSF None Detected

3000059 Coccidioides Antibodies by Complement Fixation, CSF Less than 1:2
  Histoplasma M, CSF (CF) Less than 1:2
  Histoplasma Y, CSF (CF) Less than 1:2
  Blastomyces by Immunodiffusion, CSF None detected

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Modified FDA


Negative fungal serology does not rule out the possibility of current infection. If Blastomyces antibodies are equivocal or positive by EIA then Blastomyces Immunodiffusion will be added. Additional charges apply.

Hotline History


CPT Codes

86606; 86612; 86635; 86698 x2; if reflexed, add 86612


Component Test Code* Component Chart Name LOINC
0050711 Coccidioides Ab by CF, CSF 13917-0
0050751 Aspergillus Antibody, CSF (CF) 23953-3
0050753 Histoplasma M, CSF (CF) 27220-3
0050754 Histoplasma Y, CSF (CF) 27209-6
3000233 Blastomyces Antibody by EIA, CSF 13201-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Aspergillus Antibody, CSF (CF)
  • Blastomyces Antibody by CF, CSF
  • Coccidioides Ab by CF, CSF
  • Histoplasma M, CSF (CF)
  • Histoplasma Y, CSF (CF)
Fungal Antibodies with Reflex to Blastomyces dermatitidis Antibodies by Immunodiffusion, CSF