The patient information provided with the Sequential, Specimen #1 will be used to calculate the risk

Ordering Recommendation

Second-trimester screening test for trisomy 21 (Down syndrome), trisomy 18, and open neural tube defects. Requires a previously submitted first-trimester specimen, Maternal Screening, Sequential, Specimen #1, hCG, PAPP-A, NT (3000146). Also requires nuchal translucency measurement performed by an ultrasonographer certified by the Fetal Medicine Foundation (FMF) or Nuchal Translucency Quality Review (NTQR). Risks provided in both first and second trimesters.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Specimen must be drawn between 14 weeks, 0 days and 24 weeks, 6 days gestation. The recommended time for maternal serum screening is 16 to 18 weeks gestation.


Serum Separator Tube (SST) or Plain Red.

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 3 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature


Unacceptable Conditions

Plasma. Hemolyzed specimens.


Requires that a previous first trimester specimen, Maternal Screening, Sequential, Specimen #1, hCG, PAPP-A, NT (ARUP test code 3000146), has been performed.


After separation from cells: Ambient: 72 hours; Refrigerated: 2 weeks; Frozen: 1 year (Avoid repeated freeze/thaw cycles.)


Quantitative Chemiluminescent Immunoassay




2-4 days

Reference Interval

By report

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


This test is used to screen for fetal risk of Down syndrome (trisomy 21), trisomy 18, and Open Neural Tube Defect (ONTD, spina bifida).

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0080021 Patient's AFP 1834-1
0080267 MoM for DIA 35738-4
0080268 Patient's DIA 23883-2
0080918 MoM for AFP 20450-3
0080920 Maternal Screen Interpretation 49586-1
0080922 Specimen 19151-0
0080924 Insulin Req Maternal Diabetes 44877-9
0080925 Family Hx Neural Tube Defect 8670-2
0080926 Maternal Race 21484-1
0080927 Number of Fetuses 11878-6
0080932 Maternal Age At Delivery 21612-7
0080941 Patient's uE3 2250-9
0080943 MoM for uE3 20466-9
0081065 Nuchal Translucency (NT) 12146-7
0081068 MoM for PAPP-A 32123-2
0081069 Sonographer Certification # 49089-6
0081070 Sonographer Name 49088-8
0081071 Ultrasound Date 34970-4
0081074 MoM for NT 49035-9
0081158 Family History of Aneuploidy 32435-0
3000163 PAPP-A Maternal 48407-1
3000165 Smoking 64234-8
3000166 Crown Rump Length 11957-8
3000168 Nuchal Translucency (NT), Twin B 12146-7
3000169 MoM for NT, Twin B 49035-9
3000170 Crown Rump Length, Twin B 11957-8
3000174 EER Maternal Serum, Sequential, Spcm2 11526-1
3000261 Patient's hCG, 2nd Trimester 19080-1
3000262 hCG MoM, 2nd Trimester 20465-1
3000264 Maternal Weight 29463-7
3000390 Gestational Age for Second Specimen 18185-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Maternal Serum Sequential Screen
  • second trimester screen
  • Sequential Screening
  • Serum Stepwise Sequential Screen
  • Stepwise Maternal Screen
  • Stepwise Sequential, Contingent
Maternal Screening, Sequential, Specimen #2, Alpha Fetoprotein, hCG, Estriol, and Inhibin A