Ordering Recommendation

Second-trimester screening test for open neural tube defects. Order this test for PREGNANT FEMALE patients only. For males or non-pregnant females, refer to Alpha Fetoprotein, Serum (Tumor Marker) (0080428).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Specimen must be drawn between 14 weeks, 0 days and 24 weeks, 6 days gestation.


Serum Separator Tube (SST) or Plain Red.

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature


Unacceptable Conditions

Plasma. Hemolyzed specimens.


Submit with Order: Patient's date of birth, current weight, due date, dating method (US, LMP), number of fetuses present, patient's race, if the patient was diabetic at the time of conception, if there is a known family history of neural tube defects, if the patient is currently smoking, if the patient is taking valproic acid or carbamazepine (Tegretol), if this is a repeat sample, and the age of the egg donor if an in vitro fertilization.


After separation from cells: Ambient: 72 hours; Refrigerated: 2 weeks; Frozen: 1 year (Avoid repeated freeze/thaw cycles.)


Quantitative Chemiluminescent Immunoassay




2-3 days

Reference Interval

By report

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Modified FDA


This test is used to screen for fetal risk of Open Neural Tube Defect (i.e., spina bifida).

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
0080021 Patient's AFP 1834-1
0080241 Estimated Due Date 11778-8
0080918 MoM for AFP 20450-3
0080920 Maternal Screen Interpretation 49586-1
0080922 Specimen 19151-0
0080923 Dating 21299-3
0080924 Insulin Req Maternal Diabetes 44877-9
0080925 Family Hx Neural Tube Defect 8670-2
0080926 Maternal Race 21484-1
0080927 Number of Fetuses 11878-6
0080932 Maternal Age At Delivery 21612-7
3000165 Smoking 64234-8
3000263 Gestational Age Calculated at Collection 18185-9
3000264 Maternal Weight 29463-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • AFP
  • AFP Maternal Screening
  • AFP Neural Tube Defects
  • Maternal Screening, AFP Single Marker
Maternal Serum Screen, Alpha Fetoprotein