Ordering Recommendation

Evaluate possibility of a fetal open neural tube defect at 13-36 weeks of gestation.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Amniocentesis. Specimen must be drawn between 13 weeks, 0 days and 36 weeks, 6 days gestation.


Amniotic fluid.

Specimen Preparation

Transport 2.5 mL amniotic fluid. (Min: 1.5 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Submit with Order: Gestational age at time of collection or estimated due date.


Ambient: 1 month; Refrigerated: 3 months; Frozen: 3 months


Quantitative Chemiluminescent Immunoassay/Electrophoresis




3-4 days
Reflex: 3-11 days

Reference Interval

Test Number
Reference Interval
  AFP, AF MoM ≤1.99

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Modified FDA


Information must include weeks of gestation. If the AFP (amniotic fluid) is elevated, then Acetylcholinesterase will be added. Additional charges apply. Acetylcholinesterase testing requires an additional 3-11 days to be reported.

Hotline History


CPT Codes

82106; if reflexed, add 82013 and 83033


Component Test Code* Component Chart Name LOINC
0080015 AFP, Amniotic Fluid 1832-5
0080929 AFP AF Interpretation 41273-4
3000162 AFP, AF MoM 29595-6
3000263 Gestational Age Calculated at Collection 18185-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • AFP
  • AFP-AF (Alpha-Fetoprotein, Amniotic Fluid)
  • Alpha Fetoprotein, Amniotic Fluid
  • Alpha-Fetoprotein, Amniotic Fluid
  • Fetoprotein, Amniotic Fluid
Alpha Fetoprotein (Amniotic Fluid) with Reflex to Acetylcholinesterase and Fetal Hemoglobin