Alpha Fetoprotein (Amniotic Fluid) with Reflex to Acetylcholinesterase and Fetal Hemoglobin

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Patient History Form for Prenatal Cytogenetics

Ordering Recommendation

Evaluate possibility of a fetal open neural tube defect at 13-36 weeks of gestation.

Mnemonic

AF AFP

Methodology

Quantitative Chemiluminescent Immunoassay/Electrophoresis

Performed

Sun-Sat

Reported

3-4 days
Reflex: 3-11 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Amniocentesis. Specimen must be drawn between 13 weeks, 0 days and 36 weeks, 6 days gestation.

Collect

Amniotic fluid.

Specimen Preparation

Transport 2.5 mL amniotic fluid. (Min: 1.5 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions
Remarks

Submit with Order: Gestational age at time of collection or estimated due date.

Stability

Ambient: 1 month; Refrigerated: 3 months; Frozen: 3 months

Reference Interval

Test Number
 Components
 Reference Interval
  AFP, AF MoM ≤1.99

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Modified FDA

Note

Information must include weeks of gestation. If the AFP (amniotic fluid) is elevated, then Acetylcholinesterase will be added. Additional charges apply. Acetylcholinesterase testing requires an additional 3-11 days to be reported.

Hotline History

N/A

CPT Codes

82106; if reflexed, add 82013 and 83033

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Components

Component Test Code* Component Chart Name LOINC
0080015 AFP, Amniotic Fluid 1832-5
0080929 AFP AF Interpretation 41273-4
3000162 AFP, AF MoM 29595-6
3000263 Gestational Age Calculated at Collection 18185-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • AFP
  • AFP-AF (Alpha-Fetoprotein, Amniotic Fluid)
  • Alpha Fetoprotein, Amniotic Fluid
  • Alpha-Fetoprotein, Amniotic Fluid
  • Fetoprotein, Amniotic Fluid
Alpha Fetoprotein (Amniotic Fluid) with Reflex to Acetylcholinesterase and Fetal Hemoglobin