Ordering Recommendation
Use to detect and quantitate NPM1 mutant transcripts (type A, B, and D). Use to monitor for minimal residual disease and assess the risk of disease relapse.
Mnemonic
Methodology
Quantitative Reverse Transcription Polymerase Chain Reaction
Performed
Varies
Reported
5-9 days
New York DOH Approval Status
Specimen Required
Whole blood or bone marrow in lavender (EDTA).
Whole Blood: Transport 5 mL whole blood. (Min: 3 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Refrigerate immediately. Specimens must be received within 48 hours of collection due to lability of RNA.
Whole Blood or Bone Marrow: CRITICAL REFRIGERATED. Separate specimens must be submitted when multiple tests are ordered.
Serum, plasma, ambient or frozen bone marrow, or whole blood, CSF, or FFPE tissue. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.
Ambient whole blood and ambient bone marrow specimens past 7 days will be canceled. Refrigerated whole blood or bone marrow past 7 days will be canceled.
Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable
Reference Interval
Interpretive Data
Refer to report.
Laboratory Developed Test (LDT)
Note
Hotline History
Hotline History
CPT Codes
81310
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
3000067 | NPM1 Quantitative, Source | 31208-2 |
3000068 | NPM1 Quantitative, Result | 54448-6 |
3000069 | NPM1 Quantitative, Ratio |