Aid in diagnosis of coccidioidal meningitis (Valley fever).
Semi-Quantitative Complement Fixation/Qualitative Immunodiffusion/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Transfer 2.5 mL CSF to an ARUP Standard Transport Tube. (Min: 1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens.
Other body fluids. Contaminated, hemolyzed, xanthochromic, or severely lipemic specimens.
Mark specimens plainly as "acute" or "convalescent."
Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|3000058||Coccidioides immitis by Immunodiffusion, CSF (INACTIVE as of 11/16/20: Refer to 3000061)||None detected.|
|3000059||Coccidioides Antibody by CF, CSF||Less than 1:2|
|3000055||Coccidioides Antibody IgG ELISA, CSF||
|3000056||Coccidioides Antibody IgM ELISA, CSF||
Refer to report.
Laboratory Developed Test (LDT)
For Coccidioides, immunodiffusion (ID) measures IgM antibody, while complement fixation (CF) measures both IgG and IgM. ELISA tests can be used to detect both coccidioidal IgG and IgM antibodies. While elevated single antibody titers may be diagnostic, paired specimens are preferred. Acute and convalescent specimens (drawn at least 21 days apart), showing a fourfold or greater rise in titer, are diagnostic.
Negative fungal serology does not rule out the possibility of current infection.
|Component Test Code*||Component Chart Name||LOINC|
|0050711||Coccidioides Ab by CF, CSF||13917-0|
|3000062||Coccidioides Antibody IgG ELISA, CSF||16638-9|
|3000063||Coccidioides Antibody IgM ELISA, CSF||16639-7|
|3000064||Coccidioides by Immunodiffusion, CSF||21209-2|